Mouse Rapgef2 ELISA Kit

Alternative name
Mouse cyclic nucleotide ras GEF ELISA Kit;Mouse CNRasGEF ELISA Kit;Mouse neural RAP guanine nucleotide exchange protein ELISA Kit;Mouse nRap GEP ELISA Kit;Mouse PDZ domain-containing guanine nucleotide exchange factor 1 ELISA Kit;Mouse PDZ-GEF1 ELISA Kit;Mouse RA-GEF-1 ELISA Kit;Mouse ras/Rap1-associating GEF-1 ELISA Kit;Mouse Kiaa0313 ELISA Kit;Mouse Pdzgef1 ELISA Kit;Mouse 5830453M24Rik ELISA Kit;Mouse mKIAA0313 ELISA Kit;Mouse nRapGEP ELISA Kit;Mouse Rap guanine nucleotide exchange factor (GEF) 2 ELISA Kit;Mouse rap guanine nucleotide exchange factor 2 ELISA Kit;
Catalog
E065892
species
Mouse
GeneRapgef2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse RAPGEF2. No significant cross-reactivity or interference between Mouse RAPGEF2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse RAPGEF2 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Rapgef2 ELISA Kit allows for the in vitro quantitative determination of Rapgef2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RAPGEF2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RAPGEF2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse RAPGEF2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RAPGEF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse RAPGEF2. You can calculate the concentration of Mouse RAPGEF2 in the samples by comparing the OD of the samples to the standard curve.
Mouse Rap guanine nucleotide exchange factor 2 Protein information
Uniprot ID RPGF2_MOUSE
Uniprot AC Q8CHG7; E9QMD0;
UniGene Mm.31220; Mm.462396;
GeneID 76089
Mouse Rap guanine nucleotide exchange factor 2 Protein SEQUENCE
SEQUENCE 1496 AA; 166415 MW; C2FDE48E5E21A86F CRC64;
MKPLAAPANH GVLGQQEKQS LPADFTKLHL TDSLHPQVTH VSSSHSGCSI
TSDSGSSSLS DIYQATESEA GDMDLSGLPE TAVDSEDDDD EEDIERASDP
LMSRDIVRDC LEKDPIDRTD DDIEQLLEFM HQLPAFANMT MSVRRELCAV
MVFAVVERAG TIVLNDGEEL DSWSVILNGS VEVTYPDGKA EILCMGNSFG
VSPTMDKEYM KGVMRTKVDD CQFVCIAQQD YCRILNQVEK NMQKVEEEGE
IVMVKEHREL DRTGTRKGHI VIKGTSERLT MHLVEEHSVV DPTFIEDFLL
TYRTFLSSPM EVGKKLLEWF NDPSLRDKVT RVVLLWVNNH FNDFEGDPAM
TRFLEEFENN LEREKMGGHL RLLNIACAAK AKRRLMTLTK PSREAPLPFI
LLGGSEKGFG IFVDSVDSCS KATEAGLKRG DQILEVNGQN FENIQLSKAM
EILRNNTHLS ITVKTNLFVF KELLTRLSEE KRNGAPHLPK IGDIKKASRY
SIPDLAVDVE QVIGLEKVNK KSKANTVGGR NKLKKILDKT RISILPQKPY
NDIGIGQSQD DSIVGLRQTK HIPAALPVSG TLSSSNPDLL QSHHRILDFS
TTPDLPDQVL RVFKADQQSR YIMISKDTTA KEVVIQAIRE FAVTATPEQY
SLCEVSVTPE GVIKQRRLPD QLSKLADRIQ LSGRYYLKNN METETLCSDE
DAQELLRESQ ISLLQLSTVE VATQLSMRNF ELFRNIEPTE YIDDLFKLKS
KTSCANLKKF EEVINQETFW VASEILRETN QLKRMKIIKH FIKIALHCRE
CKNFNSMFAI ISGLNLAPVA RLRTTWEKLP NKYEKLFQDL QDLFDPSRNM
AKYRNVLSGQ NLQPPVIPLF PVIKKDLTFL HEGNDSKVDG LVNFEKLRMI
AKEIRHVGRM ASVNMDPALM FRTRKKKWRS LGSLSQGSAN ATVLDVAQTG
GHKKRVRRSS FLNAKKLYED AQMARKVKQY LSNLELEMDE ESLQTLSLQC
EPATSTLPKN PGDKKPVKSE TSPVAPRAGP QQKVQPQQPL AQPQPPHKVS
QGLQVPAVSL YPSRKKVPVK DLPPFGINSP QALKKILSLS EEGSLERHRK
QAEDTISNAS SQLSSPPTSP QSSPRKGYAL ALSGTVDNFS DSGHSEISSR
SSIVSNSSFD SVPVSLHDER RQRHSVSIVE SNLGVGRMER RTLMEPDQYS
LGSYAPVSES RGLYAAATVI SSPSTEELSH DQGDRASLDA ADSGRGSWTS
CSSGSHDNIQ TIQHQRSWET LPFGHTHFDY SGDAASIWAS GGHMDQMMFS
DHSTKYNRQN QSRESLEQAQ SRASWASSTG YWGEDSEGDT GTIKRRGGKD
VSAEAESSSM VPVTTEEAKP VPMPAHIAVT PSTTKGLIAR KEGRYREPPP
TPPGYVGIPI ADFPEGPCHP ARKPPDYNVA LQRSRMVARP TEAPAPGQTP
PAAAASRPGS KPQWHKPSDA DPRLAPFQPQ GFAGAEEDED EQVSAV
UCSC uc008pnl.1; mouse.;

Category

Mouse Rapgef2 ELISA Kit