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Alternative name
Tumor necrosis factor ligand superfamily member 11 ELISA KIT; Osteoclast differentiation factor ELISA KIT; ODF ELISA KIT; Osteoprotegerin ligand ELISA KIT; OPGL ELISA KIT; Receptor activator of nuclear factor kappa-B ligand ELISA KIT; RANKL ELISA KIT; TNF-related activation-induced cytokine ELISA KIT; TRANCE ELISA KIT; CD_antigen: CD254Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 11, membrane form ELISA KIT; Tumor necrosis factor ligand superfamily member 11, soluble formTNFSF11 ELISA KIT; OPGL ELISA KIT; RANKL ELISA KIT; TRANCE ELISA KIT; ODF ELISA KIT; OPGL ELISA KIT; RANKL ELISA KIT; TRANCE ELISA KIT
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Catalog
E066030
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species
Mouse
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GeneRANKL
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SpecificityThis assay has high sensitivity and excellent specificity for detection of RANKL. No significant cross-reactivity or interference between RANKL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RANKL and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse RANKL ELISA Kit allows for the in vitro quantitative determination of RANKL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalPrinciple of the assay: RANKL ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for RANKL. Standards or samples are then added to the microtiter plate wells and RANKL if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of RANKL present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for RANKL are added to each well to "sandwich" the RANKL immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RANKL and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RANKL concentration in each sample is interpolated from this standard curve.
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