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Alternative name
Regenerating islet-derived protein 3-beta ELISA KIT; Pancreatitis-associated protein 1 ELISA KIT; Peptide 23 ELISA KIT; REG-2 ELISA KIT; Regenerating islet-derived protein III-beta ELISA KIT; Reg III-betaReg3b ELISA KIT; Pap ELISA KIT; Pap1 ELISA KIT; Reg2 ELISA KIT; REG-3-beta ELISA KIT; Reg III-beta ELISA KIT
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Catalog
E066114
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species
Mouse
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GeneReg3beta
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<0.234ng/mL
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Intended UseMouse Reg3beta ELISA Kit allows for the in vitro quantitative determination of Reg3beta , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Reg3beta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Reg3beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Reg3beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Reg3beta can be calculated.
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