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Alternative name
C-C motif chemokine 5 ELISA KIT; EoCP ELISA KIT; Eosinophil chemotactic cytokine ELISA KIT; SIS-delta ELISA KIT; Small-inducible cytokine A5 ELISA KIT; T cell-specific protein P228 ELISA KIT; TCP228CCL5 ELISA KIT; D17S136E ELISA KIT; SCYA5 ELISA KIT; TCP228 ELISA KIT
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Catalog
E066125
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species
Mouse
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GeneRANTES
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 9.4pg/ml
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Intended UseMouse RANTES ELISA Kit allows for the in vitro quantitative determination of RANTES , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Rantesantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Rantes antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Rantes amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Rantescan be calculated.
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