-
Catalog
E066214
-
species
Mouse
-
GeneRPA-1
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Intended UseMouse RPA-1 ELISA Kit allows for the in vitro quantitative determination of RPA-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: RPA-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPA-1 antibody and an RPA-1-HRP conjugate. The assay sample and buffer are incubated together with RPA-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPA-1 concentration since RPA-1 from samples and RPA-1-HRP conjugate compete for the anti-RPA-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPA-1 from the sample, fewer sites are left to bind RPA-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPA-1 concentration in each sample is interpolated from this standard curve.
-