Mouse Rtn4 ELISA Kit

Alternative name
Mouse neurite outgrowth inhibitor ELISA Kit;Mouse Nogo protein ELISA Kit;Mouse Kiaa0886 ELISA Kit;Mouse NOGO ELISA Kit;Mouse 1110020G17Rik ELISA Kit;Mouse AA407876 ELISA Kit;Mouse AA409940 ELISA Kit;Mouse AA960376 ELISA Kit;Mouse ASY ELISA Kit;Mouse C130026I10Rik ELISA Kit;Mouse NSP-CL ELISA Kit;Mouse NgA ELISA Kit;Mouse Nogo-A ELISA Kit;Mouse Nogo-B ELISA Kit;Mouse Nogo-C ELISA Kit;Mouse mKIAA0886 ELISA Kit;Mouse mKIAA4153 ELISA Kit;Mouse reticulon 4 ELISA Kit;Mouse reticulon-4 ELISA Kit;
Catalog
E066303
species
Mouse
GeneRtn4
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse RTN4. No significant cross-reactivity or interference between mouse RTN4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse RTN4 is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseMouse Rtn4 ELISA Kit allows for the in vitro quantitative determination of Rtn4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RTN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RTN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RTN4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RTN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Reticulon-4 Protein information
Uniprot ID RTN4_MOUSE
Uniprot AC Q9CTE3;
UniGene Mm.192580; Mm.440639; Mm.488364;
GeneID 68585
KEGG mmu:68585;
Mouse Reticulon-4 Protein SEQUENCE
SEQUENCE 1162 AA; 126613 MW; 855697FBEE11781F CRC64;
MEDIDQSSLV SSSADSPPRP PPAFKYQFVT EPEDEEDEED EEEEEDDEDL
EELEVLERKP AAGLSAAPVP PAAAPLLDFS SDSVPPAPRG PLPAAPPTAP
ERQPSWERSP AASAPSLPPA AAVLPSKLPE DDEPPARPPA PAGASPLAEP
AAPPSTPAAP KRRGSGSVDE TLFALPAASE PVIPSSAEKI MDLKEQPGNT
VSSGQEDFPS VLFETAASLP SLSPLSTVSF KEHGYLGNLS AVASTEGTIE
ETLNEASREL PERATNPFVN RESAEFSVLE YSEMGSSFNG SPKGESAMLV
ENTKEEVIVR SKDKEDLVCS AALHNPQESP ATLTKVVKED GVMSPEKTMD
IFNEMKMSVV APVREEYADF KPFEQAWEVK DTYEGSRDVL AARANMESKV
DKKCFEDSLE QKGHGKDSES RNENASFPRT PELVKDGSRA YITCDSFSSA
TESTAANIFP VLEDHTSENK TDEKKIEERK AQIITEKTSP KTSNPFLVAI
HDSEADYVTT DNLSKVTEAV VATMPEGLTP DLVQEACESE LNEATGTKIA
YETKVDLVQT SEAIQESIYP TAQLCPSFEE AEATPSPVLP DIVMEAPLNS
LLPSTGASVA QPSASPLEVP SPVSYDGIKL EPENPPPYEE AMSVALKTSD
SKEEIKEPES FNAAAQEAEA PYISIACDLI KETKLSTEPS PEFSNYSEIA
KFEKSVPDHC ELVDDSSPES EPVDLFSDDS IPEVPQTQEE AVMLMKESLT
EVSETVTQHK HKERLSASPQ EVGKPYLESF QPNLHITKDA ASNEIPTLTK
KETISLQMEE FNTAIYSNDD LLSSKEDKMK ESETFSDSSP IEIIDEFPTF
VSAKDDSPKE YTDLEVSNKS EIANVQSGAN SLPCSELPCD LSFKNTYPKD
EAHVSDEFSK SRSSVSKVPL LLPNVSALES QIEMGNIVKP KVLTKEAEEK
LPSDTEKEDR SLTAVLSAEL NKTSVVDLLY WRDIKKTGVV FGASLFLLLS
LTVFSIVSVT AYIALALLSV TISFRIYKGV IQAIQKSDEG HPFRAYLESE
VAISEELVQK YSNSALGHVN STIKELRRLF LVDDLVDSLK FAVLMWVFTY
VGALFNGLTL LILALISLFS IPVIYERHQA QIDHYLGLAN KSVKDAMAKI
QAKIPGLKRK AE
UCSC uc007ihn.2; mouse. [Q99P72-3];

Category

Mouse Rtn4 ELISA Kit