-
Alternative name
Somatomedin-B and thrombospondin type-1 domain-containing protein ELISA KIT; RPE-spondinSbspon ELISA KIT; Gm106 ELISA KIT; Rpesp ELISA KIT
-
Catalog
E066803
-
species
Mouse
-
GeneRpesp
-
SpecificityThis assay recognizes recombinant and natural mouse RPE-spondin. No significant cross-reactivity or interference was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Intended UseMouse Rpesp ELISA Kit allows for the in vitro quantitative determination of Rpesp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RPE-spondin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RPE-spondin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RPE-spondin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RPE-spondin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-