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Alternative name
Protein S100-A16 ELISA KIT; Aging-associated gene 13 protein ELISA KIT; Protein S100-F ELISA KIT; S100 calcium-binding protein A16S100A16 ELISA KIT; S100F ELISA KIT
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Catalog
E066896
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species
Mouse
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GeneS100A16
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SpecificityThis assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein A16 (S100A16).
No significant cross-reactivity or interference between S100 Calcium Binding Protein A16 (S100A16) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 33pg/mL.
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Intended UseMouse S100A16 ELISA Kit allows for the in vitro quantitative determination of S100A16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100 Calcium Binding Protein A16 (S100A16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to S100 Calcium Binding Protein A16 (S100A16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100 Calcium Binding Protein A16 (S100A16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of S100 Calcium Binding Protein A16 (S100A16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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