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Mouse Sfrp1 ELISA Kit

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  • Alternative name

    Mouse sFRP-1 ELISA Kit;Mouse 2210415K03Rik ELISA Kit;Mouse AW011917 ELISA Kit;Mouse AW107218 ELISA Kit;Mouse AW742929 ELISA Kit;Mouse secreted frizzled-related protein 1 ELISA Kit;Mouse secreted frizzled-related sequence protein 1 ELISA Kit;
  • Catalog
    E067129
  • species
    Mouse
  • GeneSfrp1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Secreted Frizzled Related Protein 1 (SFRP1). No significant cross-reactivity or interference between Secreted Frizzled Related Protein 1 (SFRP1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 13.3pg/mL.
  • Intended UseMouse Sfrp1 ELISA Kit allows for the in vitro quantitative determination of Sfrp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secreted Frizzled Related Protein 1 (SFRP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secreted Frizzled Related Protein 1 (SFRP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Secreted Frizzled Related Protein 1 (SFRP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Secreted frizzled-related protein 1 Protein information
  • Uniprot ID SFRP1_MOUSE
  • Uniprot AC Q8C4U3; O08861; Q505A2; Q8R1J4;
  • UniGene Mm.281691;
  • GeneID 20377
  • KEGG mmu:20377;
  • Mouse Secreted frizzled-related protein 1 Protein SEQUENCE

  • SEQUENCE 314 AA; 35413 MW; 3D98A15127DFA421 CRC64;

    MGVGRSARGR GGAASGVLLA LAAALLAAGS ASEYDYVSFQ SDIGSYQSGR

    FYTKPPQCVD IPVDLRLCHN VGYKKMVLPN LLEHETMAEV KQQASSWVPL

    LNKNCHMGTQ VFLCSLFAPV CLDRPIYPCR WLCEAVRDSC EPVMQFFGFY

    WPEMLKCDKF PEGDVCIAMT PPNTTEASKP QGTTVCPPCD NELKSEAIIE

    HLCASEFALR MKIKEVKKEN GDKKIVPKKK KPLKLGPIKK KELKRLVLFL

    KNGADCPCHQ LDNLSHNFLI MGRKVKSQYL LTAIHKWDKK NKEFKNFMKR

    MKNHECPTFQ SVFK

  • UCSC uc009lev.2; mouse.;



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