Mouse Sema3f ELISA Kit

Alternative name
Mouse semaphorin IV ELISA Kit;Mouse Sema IV ELISA Kit;Mouse Sema4 ELISA Kit;Mouse Semak ELISA Kit;Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F ELISA Kit;Mouse semaphorin-3F ELISA Kit;
Catalog
E067244
species
Mouse
GeneSema3f
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SEMA3F. No significant cross-reactivity or interference between mouse SEMA3F and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse SEMA3F is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseMouse Sema3f ELISA Kit allows for the in vitro quantitative determination of Sema3f , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Semaphorin-3F Protein information
Uniprot ID SEM3F_MOUSE
Uniprot AC O88632; E9QNC5; O88633;
UniGene Mm.12903;
GeneID 20350
KEGG mmu:20350;
Mouse Semaphorin-3F Protein SEQUENCE
SEQUENCE 785 AA; 88549 MW; 8F3F0935096C24A1 CRC64;
MLVTAFILWA SLLTGAWPAT PIQDQLPATP RVRLSFKELK ATGTAHFFNF
LLNTTDYRIL LKDEDHDRMY VGSKDYVLSL DLHDINREPL IIHWAASPQR
IEECILSGKD GNGECGNFVR LIQPWNRTHL YVCGTGAYNP MCTYVNRGRR
AQALPWTQMQ VVRGRGSRAT DGADRPTPTA PRQDYIFYLE PEKLESGKGK
CPYDPKLDTA SALINEELYA GVYIDFMGTD AAIFRTLGKQ TAMRTDQYNS
RWLNDPSFIH AELIPDSAER NDDKLYFFFR ERSAEAPQNP AVYARIGRIC
LNDDGGHCCL VNKWSTFLKA RLVCSVPGED GIETHFDELQ DVFVQQTQDV
RNPVIYAVFT SSGSVFRGSA VCVYSMADIR MVFNGPFAHK EGPNYQWMPF
SGKMPYPRPG TCPGGTFTPS MKSTKDYPDE VINFMRTHPL MYQAVYPLQR
RPLVVRTGAP YRLTTVAVDQ VDAADGRYEV LFLGTDRGTV QKVIVLPKDD
QEVEELMLEE VEVFKEPAPV KTMTISSKRQ QLYVASAVGV THLSLHRCQA
YGAACADCCL ARDPYCAWDG QACSRYTASS KRRSRRQDVR HGNPIRQCRG
FNSNANKNAV ESVQYGVAGS AAFLECQPRS PQATVKWLFQ RDPSDRRREI
RAEDRFLRTE QGLLLRALQL GDRGLYSCTA TENNFKHIVT RVQLHVLGRD
AVHAALFPPL AVSVPPPPGT GPPTPPYQEL AQLLAQPEVG LIHQYCQGYW
RHVPPRPREA PGALRPPELQ DQKKPRNRRH HPPDT
UCSC uc009rmt.1; mouse. [O88632-2];

Category

Mouse Sema3f ELISA Kit

Mouse semaphorin IV ELISA Kit;Mouse Sema IV ELISA Kit;Mouse Sema4 ELISA Kit;Mouse Semak ELISA Kit;Mouse sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F ELISA Kit;Mouse semaphorin-3F ELISA Kit;