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Mouse Sema4d ELISA Kit

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  • Alternative name

    Mouse M-sema G ELISA Kit;Mouse semaphorin-C-like 2 ELISA Kit;Mouse semaphorin-J ELISA Kit;Mouse sema J ELISA Kit;Mouse Semacl2 ELISA Kit;Mouse Semaj ELISA Kit;Mouse CD100 ELISA Kit;Mouse Semcl2 ELISA Kit;Mouse coll-4 ELISA Kit;Mouse sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D ELISA Kit;Mouse semaphorin-4D ELISA Kit;Mouse semaphorin H ELISA Kit;
  • Catalog
    E067246
  • species
    Mouse
  • GeneSema4d
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SEMA4D. No significant cross-reactivity or interference between mouse SEMA4D and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse SEMA4D is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseMouse Sema4d ELISA Kit allows for the in vitro quantitative determination of Sema4d , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA4D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA4D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA4D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA4D bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Semaphorin-4D Protein information
  • Uniprot ID SEM4D_MOUSE
  • Uniprot AC O09126; Q6GTM9;
  • UniGene Mm.33903;
  • GeneID 20354
  • KEGG mmu:20354;
  • Mouse Semaphorin-4D Protein SEQUENCE

  • SEQUENCE 861 AA; 95640 MW; B962D4E4CF37FFD9 CRC64;

    MRMCAPVRGL FLALVVVLRT AVAFAPVPRL TWEHGEVGLV QFHKPGIFNY

    SALLMSEDKD TLYVGAREAV FAVNALNISE KQHEVYWKVS EDKKSKCAEK

    GKSKQTECLN YIRVLQPLSS TSLYVCGTNA FQPTCDHLNL TSFKFLGKSE

    DGKGRCPFDP AHSYTSVMVG GELYSGTSYN FLGSEPIISR NSSHSPLRTE

    YAIPWLNEPS FVFADVIQKS PDGPEGEDDK VYFFFTEVSV EYEFVFKLMI

    PRVARVCKGD QGGLRTLQKK WTSFLKARLI CSKPDSGLVF NILQDVFVLR

    APGLKEPVFY AVFTPQLNNV GLSAVCAYTL ATVEAVFSRG KYMQSATVEQ

    SHTKWVRYNG PVPTPRPGAC IDSEARAANY TSSLNLPDKT LQFVKDHPLM

    DDSVTPIDNR PKLIKKDVNY TQIVVDRTQA LDGTFYDVMF ISTDRGALHK

    AVILTKEVHV IEETQLFRDS EPVLTLLLSS KKGRKFVYAG SNSGVVQAPL

    AFCEKHGSCE DCVLARDPYC AWSPAIKACV TLHQEEASSR GWIQDMSGDT

    SSCLDKSKES FNQHFFKHGG TAELKCFQKS NLARVVWKFQ NGELKAASPK

    YGFVGRKHLL IFNLSDGDSG VYQCLSEERV RNKTVSQLLA KHVLEVKMVP

    RTPPSPTSED AQTEGSKITS KMPVASTQGS SPPTPALWAT SPRAATLPPK

    SSSGTSCEPK MVINTVPQLH SEKTVYLKSS DNRLLMSLLL FIFVLFLCLF

    SYNCYKGYLP GQCLKFRSAL LLGKKTPKSD FSDLEQSVKE TLVEPGSFSQ

    QNGDHPKPAL DTGYETEQDT ITSKVPTDRE DSQRIDELSA RDKPFDVKCE

    LKFADSDADG D

  • UCSC uc007qmn.2; mouse.;



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