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Mouse Sema7a ELISA Kit

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  • Alternative name

    Mouse semaphorin-K1 ELISA Kit;Mouse sema K1 ELISA Kit;Mouse semaphorin-L ELISA Kit;Mouse sema L ELISA Kit;Mouse Cd108 ELISA Kit;Mouse Semal ELISA Kit;Mouse Semk1 ELISA Kit;Mouse 2900057C09Rik ELISA Kit;Mouse CDw108 ELISA Kit;Mouse H-Sema-L ELISA Kit;Mouse M-Sema-L ELISA Kit;Mouse sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A ELISA Kit;Mouse semaphorin-7A ELISA Kit;Mouse H-Sema K1 ELISA Kit;Mouse Semaphorin K1 ELISA Kit;
  • Catalog
    E067254
  • species
    Mouse
  • GeneSema7a
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse SEMA7A. No significant cross-reactivity or interference between Mouse SEMA7A and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse SEMA7A and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseMouse Sema7a ELISA Kit allows for the in vitro quantitative determination of Sema7a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA7A in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SEMA7A. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse SEMA7A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SEMA7A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse SEMA7A. You can calculate the concentration of Mouse SEMA7A in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Semaphorin-7A Protein information
  • Uniprot ID SEM7A_MOUSE
  • Uniprot AC Q9QUR8; O88371;
  • UniGene Mm.335187;
  • GeneID 20361
  • KEGG mmu:20361;
  • Mouse Semaphorin-7A Protein SEQUENCE

  • SEQUENCE 664 AA; 74994 MW; 1804A31C3C55B715 CRC64;

    MTPPPPGRAA PSAPRARVLS LPARFGLPLR LRLLLVFWVA AASAQGHSRS

    GPRISAVWKG QDHVDFSQPE PHTVLFHEPG SFSVWVGGRG KVYHFNFPEG

    KNASVRTVNI GSTKGSCQDK QDCGNYITLL ERRGNGLLVC GTNARKPSCW

    NLVNDSVVMS LGEMKGYAPF SPDENSLVLF EGDEVYSTIR KQEYNGKIPR

    FRRIRGESEL YTSDTVMQNP QFIKATIVHQ DQAYDDKIYY FFREDNPDKN

    PEAPLNVSRV AQLCRGDQGG ESSLSVSKWN TFLKAMLVCS DAATNRNFNR

    LQDVFLLPDP SGQWRDTRVY GVFSNPWNYS AVCVYSLGDI DRVFRTSSLK

    GYHMGLPNPR PGMCLPKKQP IPTETFQVAD SHPEVAQRVE PMGPLKTPLF

    HSKYHYQKVV VHRMQASNGE TFHVLYLTTD RGTIHKVVES GDQDHSFVFN

    IMEIQPFHRA AAIQAISLDA DRRKLYVTSQ WEVSQVPLDM CEVYSGGCHG

    CLMSRDPYCG WDQDRCVSIY SSQRSVLQSI NPAEPHRECP NPKPDEAPLQ

    KVSLARNSRY YLTCPMESRH ATYLWRHEEN VEQSCEPGHQ SPSCILFIEN

    LTARQYGHYR CEAQEGSYLR EAQHWELLPE DRALAEQLMG HARALAASFW

    LGVLPTLILG LLVH

  • UCSC uc009pvy.1; mouse.;



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