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Alternative name
Mouse AV098916 ELISA Kit;Mouse Saa-3 ELISA Kit;Mouse l7R3 ELISA Kit;Mouse serum amyloid A 3 ELISA Kit;Mouse serum amyloid A-3 protein ELISA Kit;
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Catalog
E067522
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species
Mouse
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GeneSaa3
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SpecificityThis assay has high sensitivity and excellent specificity for detection of SAA3. No significant cross-reactivity or interference between SAA3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SAA3 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 mug/mL.
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Intended UseMouse Saa3 ELISA Kit allows for the in vitro quantitative determination of Saa3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Categories/FamilyCardiovascular
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Product Description
specificalPrinciple of the assay: SAA3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAA3 antibody and an SAA3-HRP conjugate. The assay sample and buffer are incubated together with SAA3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAA3 concentration since SAA3 from samples and SAA3-HRP conjugate compete for the anti-SAA3 antibody binding site. Since the number of sites is limited, as more sites are occupied by SAA3 from the sample, fewer sites are left to bind SAA3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAA3 concentration in each sample is interpolated from this standard curve.
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Mouse Serum amyloid A-3 protein Protein information
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Uniprot ID
SAA3_MOUSE
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Uniprot AC
P04918; Q62201;
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UniGene
Mm.14277;
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GeneID
20210
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KEGG
mmu:20210;
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Mouse Serum amyloid A-3 protein Protein SEQUENCE
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SEQUENCE 122 AA; 13774 MW; B9435F82D1B0B705 CRC64;
MKPSIAIILC ILILGVDSQR WVQFMKEAGQ GSRDMWRAYS DMKKANWKNS
DKYFHARGNY
DAARRGPGGA WAAKVISDAR EAVQKFTGHG AEDSRADQFA
NEWGRSGKDP NHFRPAGLPK
RY
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UCSC
uc009gyw.1; mouse.;
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