Human COMP ELISA Kit

Alternative name
Human COMP ELISA Kit;Human thrombospondin-5 ELISA Kit;Human TSP5 ELISA Kit;Human EDM1 ELISA Kit;Human EPD1 ELISA Kit;Human MED ELISA Kit;Human PSACH ELISA Kit;Human THBS5 ELISA Kit;Human cartilage oligomeric matrix protein ELISA Kit;Human cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple) ELISA Kit;Human pseudoachondroplasia (epiphyseal dysplasia 1, multiple) ELISA Kit;
Catalog
E006781
species
Human
GeneCOMP
SpecificityThis assay has high sensitivity and excellent specificity for detection of COMP. No significant cross-reactivity or interference between COMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COMP and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman COMP ELISA Kit allows for the in vitro quantitative determination of COMP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This COMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human COMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||COMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-COMP antibody and an COMP-HRP conjugate. The assay sample and buffer are incubated together with COMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COMP concentration since COMP from samples and COMP-HRP conjugate compete for the anti-COMP antibody binding site. Since the number of sites is limited, as more sites are occupied by COMP from the sample, fewer sites are left to bind COMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COMP concentration in each sample is interpolated from this standard curve.
Human Cartilage oligomeric matrix protein Protein information
Uniprot ID COMP_HUMAN
Uniprot AC P49747; B4DKJ3; O14592; Q16388; Q16389; Q2NL86; Q8N4T2;
UniGene Hs.1584;
GeneID 1311
KEGG hsa:1311;
Human Cartilage oligomeric matrix protein Protein SEQUENCE
SEQUENCE 757 AA; 82860 MW; A0B73AADB39FBC7B CRC64;
MVPDTACVLL LTLAALGASG QGQSPLGSDL GPQMLRELQE TNAALQDVRE
LLRQQVREIT FLKNTVMECD ACGMQQSVRT GLPSVRPLLH CAPGFCFPGV
ACIQTESGAR CGPCPAGFTG NGSHCTDVNE CNAHPCFPRV RCINTSPGFR
CEACPPGYSG PTHQGVGLAF AKANKQVCTD INECETGQHN CVPNSVCINT
RGSFQCGPCQ PGFVGDQASG CQRRAQRFCP DGSPSECHEH ADCVLERDGS
RSCVCAVGWA GNGILCGRDT DLDGFPDEKL RCPERQCRKD NCVTVPNSGQ
EDVDRDGIGD ACDPDADGDG VPNEKDNCPL VRNPDQRNTD EDKWGDACDN
CRSQKNDDQK DTDQDGRGDA CDDDIDGDRI RNQADNCPRV PNSDQKDSDG
DGIGDACDNC PQKSNPDQAD VDHDFVGDAC DSDQDQDGDG HQDSRDNCPT
VPNSAQEDSD HDGQGDACDD DDDNDGVPDS RDNCRLVPNP GQEDADRDGV
GDVCQDDFDA DKVVDKIDVC PENAEVTLTD FRAFQTVVLD PEGDAQIDPN
WVVLNQGREI VQTMNSDPGL AVGYTAFNGV DFEGTFHVNT VTDDDYAGFI
FGYQDSSSFY VVMWKQMEQT YWQANPFRAV AEPGIQLKAV KSSTGPGEQL
RNALWHTGDT ESQVRLLWKD PRNVGWKDKK SYRWFLQHRP QVGYIRVRFY
EGPELVADSN VVLDTTMRGG RLGVFCFSQE NIIWANLRYR CNDTIPEDYE
THQLRQA
UCSC uc002nke.4; human. [P49747-1];

Category

Human COMP ELISA Kit