Mouse Smn1 ELISA Kit

Alternative name
Mouse Smn ELISA Kit;Mouse AI849087 ELISA Kit;Mouse Gemin1 ELISA Kit;Mouse survival motor neuron 1 ELISA Kit;Mouse survival motor neuron protein ELISA Kit;Mouse survival of motor neuron protein ELISA Kit;
Catalog
E067994
species
Mouse
GeneSmn1
SpecificityThis kit recognizes natural and recombinant Mouse SMN1. No significant cross-reactivity or interference between Mouse SMN1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of Mouse SMN1 is 0.938ng/mL (The sensitivity of this assay, or lowest detectable limit (LDL) was defined as the lowest protein concentration that could be differentiated from zero).
Intended UseMouse Smn1 ELISA Kit allows for the in vitro quantitative determination of Smn1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SMN1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SMN1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SMN1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of SMN1. You can calculate the concentration of SMN1 in the samples by comparing the OD of the samples to the standard curve.
Mouse Survival motor neuron protein Protein information
Uniprot ID SMN_MOUSE
Uniprot AC P97801; O09092;
UniGene Mm.2025;
GeneID 20595
KEGG mmu:20595;
Mouse Survival motor neuron protein Protein SEQUENCE
SEQUENCE 288 AA; 31254 MW; 757B3074649F7458 CRC64;
MAMGSGGAGS EQEDTVLFRR GTGQSDDSDI WDDTALIKAY DKAVASFKHA
LKNGDICETP DKPKGTARRK PAKKNKSQKK NATTPLKQWK VGDKCSAVWS
EDGCIYPATI TSIDFKRETC VVVYTGYGNR EEQNLSDLLS PTCEVANSTE
QNTQENESQV STDDSEHSSR SLRSKAHSKS KAAPWTSFLP PPPPMPGSGL
GPGKPGLKFN GPPPPPPLPP PPFLPCWMPP FPSGPPIIPP PPPISPDCLD
DTDALGSMLI SWYMSGYHTG YYMGFRQNKK EGKCSHTN
UCSC uc007rqh.1; mouse.;

Category

Mouse Smn1 ELISA Kit

Mouse Smn ELISA Kit;Mouse AI849087 ELISA Kit;Mouse Gemin1 ELISA Kit;Mouse survival motor neuron 1 ELISA Kit;Mouse survival motor neuron protein ELISA Kit;Mouse survival of motor neuron protein ELISA Kit;