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Human CASP4 ELISA Kit

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  • Alternative name

    Human CASP-4 ELISA Kit;Human ICE and Ced-3 homolog 2 ELISA Kit;Human ICH-2 ELISA Kit;Human ICE(rel)-II ELISA Kit;Human Mih1 ELISA Kit;Human protease TX ELISA Kit;Human ICH2 ELISA Kit;Human ICE(rel)II ELISA Kit;Human ICEREL-II ELISA Kit;Human Mih1/TX ELISA Kit;Human TX ELISA Kit;Human caspase 4 ELISA Kit;Human caspase-4 ELISA Kit;Human apoptotic cysteine protease Mih1/TX ELISA Kit;Human caspase 4, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 4, apoptosis-related cysteine protease ELISA Kit;Human protease ICH-2 ELISA Kit;
  • Catalog
    E006871
  • species
    Human
  • GeneCASP4
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CASP4 ELISA Kit allows for the in vitro quantitative determination of CASP4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical    Principle of the Assay: Casp4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for Casp4. Standards or samples are then added to the microtiter plate wells and Casp4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of Casp4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for Casp4 are added to each well to "sandwich" the Casp4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Casp4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Casp4 concentration in each sample is interpolated from this standard curve.
  • Human Caspase-4 subunit 2 Protein information
  • Uniprot ID CASP4_HUMAN
  • Uniprot AC Q7KYX7; Q9UG96;
  • UniGene Hs.138378;
  • GeneID 837
  • KEGG hsa:837;
  • Human Caspase-4 subunit 2 Protein SEQUENCE

  • SEQUENCE 377 AA; 43262 MW; DC7CCEC6E9D483EB CRC64;

    MAEGNHRKKP LKVLESLGKD FLTGVLDNLV EQNVLNWKEE EKKKYYDAKT

    EDKVRVMADS MQEKQRMAGQ MLLQTFFNID QISPNKKAHP NMEAGPPESG

    ESTDALKLCP HEEFLRLCKE RAEEIYPIKE RNNRTRLALI ICNTEFDHLP

    PRNGADFDIT GMKELLEGLD YSVDVEENLT ARDMESALRA FATRPEHKSS

    DSTFLVLMSH GILEGICGTV HDEKKPDVLL YDTIFQIFNN RNCLSLKDKP

    KVIIVQACRG ANRGELWVRD SPASLEVASS QSSENLEEDA VYKTHVEKDF

    IAFCSSTPHN VSWRDSTMGS IFITQLITCF QKYSWCCHLE EVFRKVQQSF

    ETPRAKAQMP TIERLSMTRY FYLFPGN

  • UCSC uc001pib.2; human. [P49662-1];



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