Mouse Sphk1 ELISA Kit

Alternative name
Mouse SK 1 ELISA Kit;Mouse SPK 1 ELISA Kit;Mouse 1110006G24Rik ELISA Kit;Mouse Sk1 ELISA Kit;Mouse Spk1 ELISA Kit;Mouse sphingosine kinase 1 ELISA Kit;
Catalog
E068774
species
Mouse
GeneSphk1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Sphk1 ELISA Kit allows for the in vitro quantitative determination of Sphk1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Sphingosine kinase 1 Protein information
Uniprot ID SPHK1_MOUSE
Uniprot AC Q8CI15; O88886; Q3U2E3; Q91ZN3;
UniGene Mm.20944;
GeneID 20698
KEGG mmu:20698;
Mouse Sphingosine kinase 1 Protein SEQUENCE
SEQUENCE 382 AA; 42443 MW; B791FAA58FCE3D29 CRC64;
MEPVECPRGL LPRPCRVLVL LNPQGGKGKA LQLFQSRVQP FLEEAEITFK
LILTERKNHA RELVCAEELG HWDALAVMSG DGLMHEVVNG LMERPDWETA
IQKPLCSLPG GSGNALAASV NHYAGYEQVT NEDLLINCTL LLCRRRLSPM
NLLSLHTASG LRLYSVLSLS WGFVADVDLE SEKYRRLGEI RFTVGTFFRL
ASLRIYQGQL AYLPVGTVAS KRPASTLVQK GPVDTHLVPL EEPVPSHWTV
VPEQDFVLVL VLLHTHLSSE LFAAPMGRCE AGVMHLFYVR AGVSRAALLR
LFLAMQKGKH MELDCPYLVH VPVVAFRLEP RSQRGVFSVD GELMVCEAVQ
GQVHPNYLWM VCGSRDAPSG RDSRRGPPPE EP
UCSC uc007mll.2; mouse. [Q8CI15-2];

Category

Mouse Sphk1 ELISA Kit

Mouse SK 1 ELISA Kit;Mouse SPK 1 ELISA Kit;Mouse 1110006G24Rik ELISA Kit;Mouse Sk1 ELISA Kit;Mouse Spk1 ELISA Kit;Mouse sphingosine kinase 1 ELISA Kit;