Mouse Cyp17a1 ELISA Kit

Alternative name
Mouse 17-alpha-hydroxyprogesterone aldolase ELISA Kit;Mouse CYPXVII ELISA Kit;Mouse cytochrome P450 17A1 ELISA Kit;Mouse cytochrome P450-C17 ELISA Kit;Mouse cytochrome P450c17 ELISA Kit;Mouse Cyp17 ELISA Kit;Mouse p450c17 ELISA Kit;Mouse cytochrome P450, family 17, subfamily a, polypeptide 1 ELISA Kit;Mouse steroid 17-alpha-hydroxylase/17,20 lyase ELISA Kit;Mouse cytochrome P450, 17 ELISA Kit;Mouse cytochrome P450, 17a1 ELISA Kit;Mouse steroid 17-alpha hydroxylase ELISA Kit;
Catalog
E068969
species
Mouse
GeneCyp17a1
SpecificityThis assay has high sensitivity and excellent specificity for detection of SEMA3A. No significant cross-reactivity or interference between SEMA3A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SEMA3A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Cyp17a1 ELISA Kit allows for the in vitro quantitative determination of Cyp17a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: SEMA3A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SEMA3A antibody and an SEMA3A-HRP conjugate. The assay sample and buffer are incubated together with SEMA3A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SEMA3A concentration since SEMA3A from samples and SEMA3A-HRP conjugate compete for the anti-SEMA3A antibody binding site. Since the number of sites is limited, as more sites are occupied by SEMA3A from the sample, fewer sites are left to bind SEMA3A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA3A concentration in each sample is interpolated from this standard curve.
Mouse Steroid 17-alpha-hydroxylase/17,20 lyase Protein information
Uniprot ID CP17A_MOUSE
Uniprot AC P27786;
UniGene Mm.1262;
GeneID 13074
KEGG mmu:13074;
Mouse Steroid 17-alpha-hydroxylase/17,20 lyase Protein SEQUENCE
SEQUENCE 507 AA; 57638 MW; C0916193356EFEBD CRC64;
MWELVGLLLL ILAYFFWPKS KTPNAKFPRS LPFLPLVGSL PFLPRRGHMH
ANFFKLQEKY GPIYSLRLGT TTAVIVGHYQ LAREVLVKKG KEFSGRPQMV
TLGLLSDQGK GVAFADSSSS WQLHRKLVFS TFSLFRDDQK LEKMICQEAN
SLCDLILTYD GESRDLSTLI FKSVINIICT ICFNISFENK DPILTTIQTF
TEGIVDVLGH SDLVDIFPWL KIFPNKNLEM IKEHTKIREK TLVEMFEKCK
EKFNSESLSS LTDILIQAKM NAENNNTGEG QDPSVFSDKH ILVTVGDIFG
AGIETTSSVL NWILAFLVHN PEVKRKIQKE IDQYVGFSRT PSFNDRTHLL
MLEATIREVL RIRPVAPLLI PHKANIDSSI GEFAIPKDTH VIINLWALHH
DKNEWDQPDR FMPERFLDPT GSHLITPTPS YLPFGAGPRS CIGEALARQE
LFIFMALLLQ RFDFDVSDDK QLPCLVGDPK VVFLIDPFKV KITVRQAWKD
AQVEVST
UCSC uc008hua.2; mouse.;

Category

Mouse Cyp17a1 ELISA Kit