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Mouse Cyp21 ELISA Kit

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  • Alternative name

    Mouse 21-OHase ELISA Kit;Mouse cytochrome P-450c21 ELISA Kit;Mouse cytochrome P450 21 ELISA Kit;Mouse cytochrome P450 XXI ELISA Kit;Mouse cytochrome P450-C21 ELISA Kit;Mouse Cyp21a-1 ELISA Kit;Mouse Cyp21a1 ELISA Kit;Mouse 21-OH ELISA Kit;Mouse 21OH ELISA Kit;Mouse 21OHA ELISA Kit;Mouse 21OHB ELISA Kit;Mouse CYP21OH-A ELISA Kit;Mouse Cyp21 ELISA Kit;Mouse Cyp21-ps1 ELISA Kit;Mouse Cyp21B ELISA Kit;Mouse Cyp21a2-ps ELISA Kit;Mouse Cyp21a2ps ELISA Kit;Mouse Oh21-1 ELISA Kit;Mouse Oh21-2 ELISA Kit;Mouse cytochrome P450, family 21, subfamily a, polypeptide 1 ELISA Kit;Mouse steroid 21-hydroxylase ELISA Kit;Mouse 21-hydroxylase ELISA Kit;Mouse cytochrome P-450 ELISA Kit;Mouse cytochrome P450 hydroxylase A ELISA Kit;Mouse cytochrome P450, 21, pseudogene ELISA Kit;Mouse cytochrome P450, 21, steroid 21 hydroxylase ELISA Kit;Mouse cytochrome P450, family 21, subfamily a, polypeptide 2, pseudogene ELISA Kit;
  • Catalog
    E068972
  • species
    Mouse
  • GeneCyp21
  • SpecificityThis assay recognizes recombinant and natural mouse Steroid 21-hydroxylase. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Cyp21 ELISA Kit allows for the in vitro quantitative determination of Cyp21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Steroid 21-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Steroid 21-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Steroid 21-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Steroid 21-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Steroid 21-hydroxylase Protein information
  • Uniprot ID CP21A_MOUSE
  • Uniprot AC P03940; A0JP50; O88304; Q64510; Q9QX14;
  • UniGene Mm.440407;
  • GeneID 13079
  • KEGG mmu:13079;
  • Mouse Steroid 21-hydroxylase Protein SEQUENCE

  • SEQUENCE 487 AA; 55328 MW; CAD32B606004449F CRC64;

    MLLPGLLLLL LLLAGTRWLW GQWKLRKLHL PPLAPGFLHF LQPNLPIYLL

    GLTQKLGPIY RIRLGMQDVV VLNSNRTIEE ALIQKWVDFA GRPHMLNGKM

    DLDLSLGDYS LMWKAHKKLS RSALMLGMRD SMEPLIEQLT QEFCERMRAQ

    AGTPVAIHKE FSFLTCSIIS CLTFGDKDST LVQTLHDCVQ DLLQAWNHWS

    IQILTIIPLL RFLPNPGLQK LKQIQESRDH IVKQQLKQHK DSLVAGQWKD

    MIDYMLQGVE KQRDGKDEER LHEGHVHMSV VDLFIGGTET TATTLSWAVA

    FLLHHPEIQK RLQEELDLKL GPGSQLLYRN RMQLPLLMAT IAEVLRLRPV

    VPLALPHRAT RASSISGYDI PKDMVIIPNI QGANLDEMVW ELPSKFWPDR

    FLEPGKNPRT PSFGCGARVC LGEPLARLEL FVVLARLLQA FTLLPPPDGT

    LPSLQPQPYA GINLPIPPFQ VRLQPRNLAP QDQGERP

  • UCSC uc008cdo.2; mouse.;



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