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Mouse Srd5a1 ELISA Kit

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  • Alternative name

    Mouse SR type 1 ELISA Kit;Mouse Steroid 5-alpha-reductase 1 ELISA Kit;Mouse S5AR 1 ELISA Kit;Mouse 0610031P22Rik ELISA Kit;Mouse 4930435F02Rik ELISA Kit;Mouse Srd5a-1 ELISA Kit;Mouse steroid 5 alpha-reductase 1 ELISA Kit;Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 1 ELISA Kit;Mouse type 1 5alpha-reductase ELISA Kit;
  • Catalog
    E068974
  • species
    Mouse
  • GeneSrd5a1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SRD5a1. No significant cross-reactivity or interference between SRD5a1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of SRD5a1 is typically less than 0.058ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding t
  • Intended UseMouse Srd5a1 ELISA Kit allows for the in vitro quantitative determination of Srd5a1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SRD5a1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SRD5a1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SRD5a1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SRD5a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 1 Protein information
  • Uniprot ID S5A1_MOUSE
  • Uniprot AC Q68FF9; Q505K7;
  • UniGene Mm.451912; Mm.483181;
  • GeneID 78925
  • KEGG mmu:78925;
  • Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 1 Protein SEQUENCE

  • SEQUENCE 255 AA; 29344 MW; 80A5624B25D46D1A CRC64;

    MELDELCLLD ALVYLEGFLA FVAFVGLQMV GSSYGRYSSQ WSGRRVPARP

    AWFLQELPSM AWPLYECIRP AAARLGNLPN RVLLAMFLIH YVQRTLVFPV

    LIRGGKPTLL FTFVLAFLFC TLNGYLQSRY LSQFAVYAED WVTHPCFLTG

    FALWLVGMVI NIHSDHILRN LRKPGETGYK IPRGGLFEYV SSANYFGELV

    EWCGFALASW SLQGVVFALF TLCALFTRAR QHHQWYLEKF EDYPKTRKIL

    IPFLL

  • UCSC uc007rck.1; mouse.;



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