Mouse Syt1 ELISA Kit

Alternative name
Mouse synaptotagmin I ELISA Kit;Mouse SytI ELISA Kit;Mouse p65 ELISA Kit;Mouse AW124717 ELISA Kit;Mouse G630098F17Rik ELISA Kit;Mouse synaptotagmin-1 ELISA Kit;
Catalog
E069329
species
Mouse
GeneSyt1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseMouse Syt1 ELISA Kit allows for the in vitro quantitative determination of Syt1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse SYT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse SYT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse SYT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse SYT1 can be calculated.
Mouse Synaptotagmin-1 Protein information
Uniprot ID SYT1_MOUSE
Uniprot AC P46096;
UniGene Mm.289702; Mm.489829;
GeneID 20979
KEGG mmu:20979;
Mouse Synaptotagmin-1 Protein SEQUENCE
SEQUENCE 421 AA; 47418 MW; 7FDEFF37170BD169 CRC64;
MVSASRPEAL AAPVTTVATL VPHNATEPAS PGEGKEDAFS KLKQKFMNEL
HKIPLPPWAL IAIAIVAVLL VVTCCFCVCK KCLFKKKNKK KGKEKGGKNA
INMKDVKDLG KTMKDQALKD DDAETGLTDG EEKEEPKEEE KLGKLQYSLD
YDFQNNQLLV GIIQAAELPA LDMGGTSDPY VKVFLLPDKK KKFETKVHRK
TLNPVFNEQF TFKVPYSELG GKTLVMAVYD FDRFSKHDII GEFKVPMNTV
DFGHVTEEWR DLQSAEKEEQ EKLGDICFSL RYVPTAGKLT VVILEAKNLK
KMDVGGLSDP YVKIHLMQNG KRLKKKKTTI KKNTLNPYYN ESFSFEVPFE
QIQKVQVVVT VLDYDKIGKN DAIGKVFVGY NSTGAELRHW SDMLANPRRP
IAQWHTLQVE EEVDAMLAVK K
UCSC uc007gzg.2; mouse.;

Category

Mouse Syt1 ELISA Kit

Mouse synaptotagmin I ELISA Kit;Mouse SytI ELISA Kit;Mouse p65 ELISA Kit;Mouse AW124717 ELISA Kit;Mouse G630098F17Rik ELISA Kit;Mouse synaptotagmin-1 ELISA Kit;