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Alternative name
Angiopoietin-1 receptor ELISA KIT; Endothelial tyrosine kinase ELISA KIT; Tunica interna endothelial cell kinase ELISA KIT; Tyrosine kinase with Ig and EGF homology domains-2 ELISA KIT; Tyrosine-protein kinase receptor TEK ELISA KIT; Tyrosine-protein kinase receptor TIE-2 ELISA KIT; hTIE2 ELISA KIT; p140 TEK ELISA KIT; CD_antigen: CD202bTEK ELISA KIT; TIE2 ELISA KIT; VMCM ELISA KIT; VMCM1 ELISA KIT; hTIE2 ELISA KIT
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Catalog
E069698
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species
Mouse
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GeneTie2
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SpecificityThis assay has high sensitivity and excellent specificity for detection of TEK Tyrosine Kinase, Endothelial (Tie2).
No significant cross-reactivity or interference between TEK Tyrosine Kinase, Endothelial (Tie2) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.058ng/mL.
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Intended UseMouse Tie2 ELISA Kit allows for the in vitro quantitative determination of Tie2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TEK Tyrosine Kinase, Endothelial (Tie2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TEK Tyrosine Kinase, Endothelial (Tie2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TEK Tyrosine Kinase, Endothelial (Tie2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TEK Tyrosine Kinase, Endothelial (Tie2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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