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Alternative name
Transforming growth factor beta-2 ELISA KIT; BSC-1 cell growth inhibitor ELISA KIT; Cetermin ELISA KIT; Glioblastoma-derived T-cell suppressor factor ELISA KIT; G-TSF ELISA KIT; PolyerginTGFB2 ELISA KIT; TGF-beta-2 ELISA KIT; G-TSF ELISA KIT; LAP ELISA KIT
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Catalog
E069903
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species
Mouse
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GeneTGFbeta2
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse TGFbeta2 ELISA Kit allows for the in vitro quantitative determination of TGFbeta2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. The purified anti-TGF- beta 2 antibody was pre-coated onto 96-well plates. And the HRP conjugated anti-TGF-beta2 antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, mixed and incubated, then, unbound conjugates were washed away with wash buffer. TMB substrates (A & B) were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TGF-beta2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TGF-beta2 can be calculated.
Background: Transforming growth factor-beta 2 (TGF-beta2) is a secreted protein known as a cytokine that performs many cellular functions and has a vital role during embryonic development. It is known to suppress the effects of interleukin dependent T-cell tumors. It is present at elevated levels in the aqueous humor of patients with primary open angle glaucoma. In 2004, Gottanka found that TGF-beta2 reduced outflow facility when perfused into cultured Mouse anterior segments. Furthermore, TGF-beta2 affected the extracellular matrix of the trabecular meshwork in a manner that was consistent with the observed reduction in outflow facility.
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