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Alternative name
Mouse trx ELISA Kit;Mouse ATL-derived factor ELISA Kit;Mouse ADF ELISA Kit;Mouse Txn1 ELISA Kit;Mouse AW550880 ELISA Kit;Mouse Trx1 ELISA Kit;Mouse Txn ELISA Kit;Mouse thioredoxin 1 ELISA Kit;Mouse thioredoxin ELISA Kit;
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Catalog
E069927
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species
Mouse
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GeneTxn
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 37.5pg/ml
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Intended UseMouse Txn ELISA Kit allows for the in vitro quantitative determination of Txn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Trx antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Trx antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Trx amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse Trx can be calculated.
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Mouse Thioredoxin Protein information
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Uniprot ID
THIO_MOUSE
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Uniprot AC
P10639; Q52KC4; Q9D8R0;
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UniGene
Mm.260618;
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GeneID
22166
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KEGG
mmu:22166;
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Mouse Thioredoxin Protein SEQUENCE
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SEQUENCE 105 AA; 11675 MW; 3A2B925935F952DA CRC64;
MVKLIESKEA FQEALAAAGD KLVVVDFSAT WCGPCKMIKP FFHSLCDKYS
NVVFLEVDVD
DCQDVAADCE VKCMPTFQFY KKGQKVGEFS GANKEKLEAS
ITEYA
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UCSC
uc008syp.1; mouse.;
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