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Human ADAM12 ELISA Kit

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  • Alternative name

    Human ADAM 12 ELISA Kit;Human meltrin-alpha ELISA Kit;Human MLTN ELISA Kit;Human ADAM12-OT1 ELISA Kit;Human CAR10 ELISA Kit;Human MCMP ELISA Kit;Human MCMPMltna ELISA Kit;Human MLTNA ELISA Kit;Human ADAM metallopeptidase domain 12 ELISA Kit;Human disintegrin and metalloproteinase domain-containing protein 12 ELISA Kit;Human metalloprotease-disintegrin 12 transmembrane ELISA Kit;
  • Catalog
    E000007
  • species
    Human
  • GeneADAM12
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 12 (ADAM12). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 12 (ADAM12) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.057ng/mL.
  • Intended UseHuman ADAM12 ELISA Kit allows for the in vitro quantitative determination of ADAM12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 12 (ADAM12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 12 (ADAM12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of A Disintegrin And Metalloprotease 12 (ADAM12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Disintegrin and metalloproteinase domain-containing protein 12 Protein information
  • Uniprot ID ADA12_HUMAN
  • Uniprot AC RDSSNSCD LPEFCTGASP HCPANVYLHD GHSCQDVDGY CYNGICQTHE QQCVTLWGPG
  • UniGene Hs.594351; Hs.741333;
  • GeneID 8038
  • KEGG hsa:8038;
  • Human Disintegrin and metalloproteinase domain-containing protein 12 Protein SEQUENCE

  • SEQUENCE 909 AA; 99542 MW; E28131C64C4304AB CRC64;

    MAARPLPVSP ARALLLALAG ALLAPCEARG VSLWNQGRAD EVVSASVGSG

    DLWIPVKSFD SKNHPEVLNI RLQRESKELI INLERNEGLI ASSFTETHYL

    QDGTDVSLAR NYTVILGHCY YHGHVRGYSD SAVSLSTCSG LRGLIVFENE

    SYVLEPMKSA TNRYKLFPAK KLKSVRGSCG SHHNTPNLAA KNVFPPPSQT

    WARRHKRETL KATKYVELVI VADNREFQRQ GKDLEKVKQR LIEIANHVDK

    FYRPLNIRIV LVGVEVWNDM DKCSVSQDPF TSLHEFLDWR KMKLLPRKSH

    DNAQLVSGVY FQGTTIGMAP IMSMCTADQS GGIVMDHSDN PLGAAVTLAH

    ELGHNFGMNH DTLDRGCSCQ MAVEKGGCIM NASTGYPFPM VFSSCSRKDL

    ETSLEKGMGV CLFNLPEVRE SFGGQKCGNR FVEEGEECDC GEPEECMNRC

    CNATTCTLKP DAVCAHGLCC EDCQLKPAGT ACRDSSNSCD LPEFCTGASP

    HCPANVYLHD GHSCQDVDGY CYNGICQTHE QQCVTLWGPG AKPAPGICFE

    RVNSAGDPYG NCGKVSKSSF AKCEMRDAKC GKIQCQGGAS RPVIGTNAVS

    IETNIPLQQG GRILCRGTHV YLGDDMPDPG LVLAGTKCAD GKICLNRQCQ

    NISVFGVHEC AMQCHGRGVC NNRKNCHCEA HWAPPFCDKF GFGGSTDSGP

    IRQADNQGLT IGILVTILCL LAAGFVVYLK RKTLIRLLFT NKKTTIEKLR

    CVRPSRPPRG FQPCQAHLGH LGKGLMRKPP DSYPPKDNPR RLLQCQNVDI

    SRPLNGLNVP QPQSTQRVLP PLHRAPRAPS VPARPLPAKP ALRQAQGTCK

    PNPPQKPLPA DPLARTTRLT HALARTPGQW ETGLRLAPLR PAPQYPHQVP

    RSTHTAYIK

  • UCSC uc001ljk.4; human. [O43184-1];



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