Mouse Tnfsf12 ELISA Kit

Alternative name
Mouse TNF-related weak inducer of apoptosis ELISA Kit;Mouse Tweak ELISA Kit;Mouse Apo3l ELISA Kit;Mouse Dr3l ELISA Kit;Mouse Dr3lg ELISA Kit;Mouse tumor necrosis factor (ligand) superfamily, member 12 ELISA Kit;Mouse tumor necrosis factor ligand superfamily member 12 ELISA Kit;
Catalog
E070334
species
Mouse
GeneTnfsf12
SpecificityNatural and recombinant mouse TNFSF12
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10 pg/ml
Intended UseMouse Tnfsf12 ELISA Kit allows for the in vitro quantitative determination of Tnfsf12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: mouse TNFSF12 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFSF12 has been precoated onto 96-well plates. Standards(E.coli, S94-H249) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFSF12 amount of sample captured in plate. Background: Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis (TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
Mouse Tumor necrosis factor ligand superfamily member 12, secreted form Protein information
Uniprot ID TNF12_MOUSE
Uniprot AC O54907; Q9CTP2;
UniGene Mm.8983;
GeneID 21944
KEGG mmu:21944;
Mouse Tumor necrosis factor ligand superfamily member 12, secreted form Protein SEQUENCE
SEQUENCE 249 AA; 27441 MW; 08352620444EFB7B CRC64;
MAARRSQRRR GRRGEPGTAL LAPLVLSLGL ALACLGLLLV VVSLGSWATL
SAQEPSQEEL TAEDRREPPE LNPQTEESQD VVPFLEQLVR PRRSAPKGRK
ARPRRAIAAH YEVHPRPGQD GAQAGVDGTV SGWEETKINS SSPLRYDRQI
GEFTVIRAGL YYLYCQVHFD EGKAVYLKLD LLVNGVLALR CLEEFSATAA
SSPGPQLRLC QVSGLLPLRP GSSLRIRTLP WAHLKAAPFL TYFGLFQVH
UCSC uc007jri.2; mouse.;

Category

Mouse Tnfsf12 ELISA Kit

Mouse TNF-related weak inducer of apoptosis ELISA Kit;Mouse Tweak ELISA Kit;Mouse Apo3l ELISA Kit;Mouse Dr3l ELISA Kit;Mouse Dr3lg ELISA Kit;Mouse tumor necrosis factor (ligand) superfamily, member 12 ELISA Kit;Mouse tumor necrosis factor ligand superfamily member 12 ELISA Kit;