Human CTSL ELISA Kit

Alternative name
Human cathepsin L ELISA Kit;Human major excreted protein ELISA Kit;Human MEP ELISA Kit;Human CTSL1 ELISA Kit;Human CATL ELISA Kit;Human cathepsin L1 ELISA Kit;
Catalog
E007061
species
Human
GeneCTSL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cathepsin L (CTSL). No significant cross-reactivity or interference between Cathepsin L (CTSL) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 27.5pg/mL.
Intended UseHuman CTSL ELISA Kit allows for the in vitro quantitative determination of CTSL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin L (CTSL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin L (CTSL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin L (CTSL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cathepsin L (CTSL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Cathepsin L1 light chain Protein information
Uniprot ID CATL1_HUMAN
Uniprot AC P07711; Q6IAV1; Q96QJ0;
UniGene Hs.731507;
GeneID 1514
KEGG hsa:1514;
Human Cathepsin L1 light chain Protein SEQUENCE
SEQUENCE 333 AA; 37564 MW; 8CD17D00EF859D85 CRC64;
MNPTLILAAF CLGIASATLT FDHSLEAQWT KWKAMHNRLY GMNEEGWRRA
VWEKNMKMIE LHNQEYREGK HSFTMAMNAF GDMTSEEFRQ VMNGFQNRKP
RKGKVFQEPL FYEAPRSVDW REKGYVTPVK NQGQCGSCWA FSATGALEGQ
MFRKTGRLIS LSEQNLVDCS GPQGNEGCNG GLMDYAFQYV QDNGGLDSEE
SYPYEATEES CKYNPKYSVA NDTGFVDIPK QEKALMKAVA TVGPISVAID
AGHESFLFYK EGIYFEPDCS SEDMDHGVLV VGYGFESTES DNNKYWLVKN
SWGEEWGMGG YVKMAKDRRN HCGIASAASY PTV
UCSC uc004aph.4; human.;

Category

Human CTSL ELISA Kit

Human cathepsin L ELISA Kit;Human major excreted protein ELISA Kit;Human MEP ELISA Kit;Human CTSL1 ELISA Kit;Human CATL ELISA Kit;Human cathepsin L1 ELISA Kit;