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Alternative name
Protein-glutamine gamma-glutamyltransferase 6 ELISA KIT; Transglutaminase Y ELISA KIT; TGY ELISA KIT; TGase Y ELISA KIT; Transglutaminase-3-like ELISA KIT; TGase-3-like ELISA KIT; Transglutaminase-6 ELISA KIT; TG6 ELISA KIT; TGase-6TGM6 ELISA KIT; TGM3L ELISA KIT; TGY ELISA KIT; TGase Y ELISA KIT; TGase-3-like ELISA KIT; TG6 ELISA KIT; TGase-6 ELISA KIT
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Catalog
E070755
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species
Mouse
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GeneTGM6
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Transglutaminase 6 (TGM6).
No significant cross-reactivity or interference between Transglutaminase 6 (TGM6) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 6.6pg/mL.
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Intended UseMouse TGM6 ELISA Kit allows for the in vitro quantitative determination of TGM6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transglutaminase 6 (TGM6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transglutaminase 6 (TGM6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transglutaminase 6 (TGM6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transglutaminase 6 (TGM6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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