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Alternative name
Human CK-beta-10 ELISA Kit;Human monocyte chemoattractant protein 4 ELISA Kit;Human monocyte chemotactic protein 4 ELISA Kit;Human MCP-4 ELISA Kit;Human NCC-1 ELISA Kit;Human small-inducible cytokine A13 ELISA Kit;Human MCP4 ELISA Kit;Human NCC1 ELISA Kit;Human SCYA13 ELISA Kit;Human CKb10 ELISA Kit;Human SCYL1 ELISA Kit;Human C-C motif chemokine ligand 13 ELISA Kit;Human C-C motif chemokine 13 ELISA Kit;Human chemokine (C-C motif) ligand 13 ELISA Kit;Human new CC chemokine 1 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 13 ELISA Kit;
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Catalog
E007186
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species
Human
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GeneCCL13
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SpecificityNatural and recombinant human CCL13
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10 pg/ml
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Intended UseHuman CCL13 ELISA Kit allows for the in vitro quantitative determination of CCL13 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL13 has been precoated onto 96-well plates. Standards(E.coli, Q24-T98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL13 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.
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Human C-C motif chemokine 13, short chain Protein information
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Uniprot ID
CCL13_HUMAN
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Uniprot AC
Q99616; O95689; Q6ICQ6;
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UniGene
Hs.414629;
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GeneID
6357
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KEGG
hsa:6357;
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Human C-C motif chemokine 13, short chain Protein SEQUENCE
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SEQUENCE 98 AA; 10986 MW; 612688DFCD308873 CRC64;
MKVSAVLLCL LLMTAAFNPQ GLAQPDALNV PSTCCFTFSS KKISLQRLKS
YVITTSRCPQ
KAVIFRTKLG KEICADPKEK WVQNYMKHLG RKAHTLKT
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UCSC
uc002hic.3; human.;
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