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Alternative name
Human Chemokine CC-4 ELISA Kit;Human HCC-4 ELISA Kit;Human chemokine LEC ELISA Kit;Human IL-10-inducible chemokine ELISA Kit;Human LCC-1 ELISA Kit;Human liver-expressed chemokine ELISA Kit;Human lymphocyte and monocyte chemoattractant ELISA Kit;Human LMC ELISA Kit;Human monotactin-1 ELISA Kit;Human Mtn-1 ELISA Kit;Human NCC-4 ELISA Kit;Human small-inducible cytokine A16 ELISA Kit;Human ILINCK ELISA Kit;Human NCC4 ELISA Kit;Human SCYA16 ELISA Kit;Human CKb12 ELISA Kit;Human LEC ELISA Kit;Human SCYL4 ELISA Kit;Human C-C motif chemokine ligand 16 ELISA Kit;Human C-C motif chemokine 16 ELISA Kit;Human chemokine (C-C motif) ligand 16 ELISA Kit;Human liver CC chemokine-1 ELISA Kit;Human new CC chemokine 4 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 16 ELISA Kit;
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Catalog
E007193
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species
Human
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GeneCCL16
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SpecificityNatural and recombinant human CCL16
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10pg/ml
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Intended UseHuman CCL16 ELISA Kit allows for the in vitro quantitative determination of CCL16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL16 has been precoated onto 96-well plates. Standards(E.coli, Q24-Q120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL16 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.
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Human C-C motif chemokine 16 Protein information
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Uniprot ID
CCL16_HUMAN
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Uniprot AC
O15467; Q4KKU0;
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UniGene
Hs.10458;
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GeneID
6360
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KEGG
hsa:6360;
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Human C-C motif chemokine 16 Protein SEQUENCE
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SEQUENCE 120 AA; 13600 MW; 373D73016134D894 CRC64;
MKVSEAALSL LVLILIITSA SRSQPKVPEW VNTPSTCCLK YYEKVLPRRL
VVGYRKALNC
HLPAIIFVTK RNREVCTNPN DDWVQEYIKD PNLPLLPTRN
LSTVKIITAK NGQPQLLNSQ
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UCSC
uc002hkl.4; human.;
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