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Alternative name
Human alternative macrophage activation-associated CC chemokine 1 ELISA Kit;Human AMAC-1 ELISA Kit;Human CC chemokine PARC ELISA Kit;Human dendritic cell chemokine 1 ELISA Kit;Human DC-CK1 ELISA Kit;Human macrophage inflammatory protein 4 ELISA Kit;Human MIP-4 ELISA Kit;Human pulmonary and activation-regulated chemokine ELISA Kit;Human Small-inducible cytokine A18 ELISA Kit;Human AMAC1 ELISA Kit;Human DCCK1 ELISA Kit;Human MIP4 ELISA Kit;Human PARC ELISA Kit;Human SCYA18 ELISA Kit;Human CKb7 ELISA Kit;Human C-C motif chemokine ligand 18 ELISA Kit;Human C-C motif chemokine 18 ELISA Kit;Human CC chemokine ligand 18 ELISA Kit;Human chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) ELISA Kit;Human chemokine (C-C), dendritic ELISA Kit;Human small inducible cytokine A18 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary and activation-regulated ELISA Kit;
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Catalog
E007198
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species
Human
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GeneCCL18
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SpecificityNatural and recombinant human CCL18
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10pg/ml
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Intended UseHuman CCL18 ELISA Kit allows for the in vitro quantitative determination of CCL18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL18 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL18 has been precoated onto 96-well plates. Standards(E.coli, A21-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL18 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL18 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine). CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.
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Human CCL18(4-69) Protein information
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Uniprot ID
CCL18_HUMAN
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Uniprot AC
P55774; B5BUM2; Q53X71;
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UniGene
Hs.143961;
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GeneID
6362
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KEGG
hsa:6362;
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Human CCL18(4-69) Protein SEQUENCE
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SEQUENCE 89 AA; 9849 MW; C287B94B9C0518E4 CRC64;
MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET
SPQCPKPGVI
LLTKRGRQIC ADPNKKWVQK YISDLKLNA
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UCSC
uc002hku.4; human.;
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