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Alternative name
Human CK-BETA-8 ELISA Kit;Human Ckb-8 ELISA Kit;Human macrophage inflammatory protein 3 ELISA Kit;Human MIP-3 ELISA Kit;Human myeloid progenitor inhibitory factor 1 ELISA Kit;Human MPIF-1 ELISA Kit;Human Small-inducible cytokine A23 ELISA Kit;Human MIP3 ELISA Kit;Human MPIF1 ELISA Kit;Human SCYA23 ELISA Kit;Human CKb8 ELISA Kit;Human Ckb-8-1 ELISA Kit;Human hmrp-2a ELISA Kit;Human C-C motif chemokine ligand 23 ELISA Kit;Human C-C motif chemokine 23 ELISA Kit;Human C6 beta-chemokine ELISA Kit;Human chemokine (C-C motif) ligand 23 ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 23 ELISA Kit;
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Catalog
E007208
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species
Human
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GeneCCL23
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SpecificityNatural and recombinant human CCL23
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 2pg/ml
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Intended UseHuman CCL23 ELISA Kit allows for the in vitro quantitative determination of CCL23 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL23 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL23 has been precoated onto 96-well plates. Standards(E.coli, R22-N120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL23 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL23 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 23 (CCL23), also known as MIP-3 and MPIF-1, is a small cytokine belonging to the CC chemokine family. It is mapped to 17q12. CCL23 is predominantly expressed in lung and liver tissue, but is also found in placenta, bone marrow and some cell lines of myeloid origin. MPIF1 has chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils. It has also been attributed to an inhibitory activity on hematopoietic progenitor cells that give rise to granulocyte and monocyte lineages. Moreover, CCL23 is a ligand for the chemokine receptor CCR1.
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Human CCL23(30-99) Protein information
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Uniprot ID
CCL23_HUMAN
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Uniprot AC
P55773; B7ZKQ3; O00174; O75950; Q52LD4;
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UniGene
Hs.169191;
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GeneID
6368
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KEGG
hsa:6368;
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Human CCL23(30-99) Protein SEQUENCE
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SEQUENCE 120 AA; 13411 MW; 6CA622DD3A4B3A1A CRC64;
MKVSVAALSC LMLVTALGSQ ARVTKDAETE FMMSKLPLEN PVLLDRFHAT
SADCCISYTP
RSIPCSLLES YFETNSECSK PGVIFLTKKG RRFCANPSDK
QVQVCVRMLK LDTRIKTRKN
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UCSC
uc002hks.3; human. [P55773-1];
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