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Alternative name
Human CC chemokine ILC ELISA Kit;Human Cutaneous T-cell-attracting chemokine ELISA Kit;Human CTACK ELISA Kit;Human ESKINE ELISA Kit;Human IL-11 R-alpha-locus chemokine ELISA Kit;Human skinkine ELISA Kit;Human small-inducible cytokine A27 ELISA Kit;Human ILC ELISA Kit;Human SCYA27 ELISA Kit;Human ALP ELISA Kit;Human CTAK ELISA Kit;Human PESKY ELISA Kit;Human C-C motif chemokine ligand 27 ELISA Kit;Human C-C motif chemokine 27 ELISA Kit;Human IL-11 Ralpha-locus chemokine ELISA Kit;Human chemokine (C-C motif) ligand 27 ELISA Kit;Human cutaneous T-cell attracting chemokine ELISA Kit;Human small inducible cytokine subfamily A (Cys-Cys), member 27 ELISA Kit;
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Catalog
E007213
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species
Human
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GeneCCL27
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SpecificityNatural and recombinant human CCL27
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity< 10pg/ml
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Intended UseHuman CCL27 ELISA Kit allows for the in vitro quantitative determination of CCL27 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the assay: human CCL27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL27 has been precoated onto 96-well plates. Standards(E.coli, F25-G112) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL27 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). This gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
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Human C-C motif chemokine 27 Protein information
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Uniprot ID
CCL27_HUMAN
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Uniprot AC
Q9Y4X3;
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UniGene
Hs.648124;
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GeneID
10850
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KEGG
hsa:10850;
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Human C-C motif chemokine 27 Protein SEQUENCE
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SEQUENCE 112 AA; 12618 MW; 7B5940E147ABF820 CRC64;
MKGPPTFCSL LLLSLLLSPD PTAAFLLPPS TACCTQLYRK PLSDKLLRKV
IQVELQEADG
DCHLQAFVLH LAQRSICIHP QNPSLSQWFE HQERKLHGTL
PKLNFGMLRK MG
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UCSC
uc003zvm.1; human.;
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