-
Alternative name
Human G0/G1 switch regulatory protein 19-1 ELISA Kit;Human macrophage inflammatory protein 1-alpha ELISA Kit;Human MIP-1-alpha ELISA Kit;Human PAT 464.1 ELISA Kit;Human SIS-beta ELISA Kit;Human Small-inducible cytokine A3 ELISA Kit;Human tonsillar lymphocyte LD78 alpha protein ELISA Kit;Human LD78-alpha(4-69) ELISA Kit;Human G0S19-1 ELISA Kit;Human MIP1A ELISA Kit;Human SCYA3 ELISA Kit;Human LD78ALPHA ELISA Kit;Human C-C motif chemokine ligand 3 ELISA Kit;Human C-C motif chemokine 3 ELISA Kit;Human chemokine (C-C motif) ligand 3 ELISA Kit;Human small inducible cytokine A3 (homologous to mouse Mip-1a) ELISA Kit;
-
Catalog
E007215
-
species
Human
-
GeneCCL3
-
SpecificityThe Human CCL3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity10 pg/ml
-
Intended UseHuman CCL3 ELISA Kit allows for the in vitro quantitative determination of CCL3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageStore the whole ELISA kit at 4℃
-
Product Description
specificalPrinciple of the Assay: The Human CCL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CCL3 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CCL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and CCL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CCL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: Macrophage inflammatory protein-1 alpha (MIP-1 alpha), also called CCL3, LD78. The cDNA for MIP-1 alpha predicts a mature peptide of 69 amino acids with a molecular mass of 7,889 daltons. LD78 is a member of a newly identified superfamily of small inducible proteins involved in inflammatory responses, wound healing and tumorigenesis. MIP-1 alpha is a chemokine that has pro-inflammatory and stem cell inhibitory activities in vitro. It constitutes an important second signal for mast cell degranulation in the conjunctiva in vivo and consequently for acute-phase disease.
-
Human MIP-1-alpha(4-69) Protein information
-
Uniprot ID
CCL3_HUMAN
-
Uniprot AC
P10147;
-
UniGene
Hs.514107;
-
GeneID
6348
-
KEGG
hsa:6348;
-
Human MIP-1-alpha(4-69) Protein SEQUENCE
-
SEQUENCE 92 AA; 10085 MW; 517865D5D6776CA8 CRC64;
MQVSTAALAV LLCTMALCNQ FSASLAADTP TACCFSYTSR QIPQNFIADY
FETSSQCSKP
GVIFLTKRSR QVCADPSEEW VQKYVSDLEL SA
-
UCSC
uc002hkv.5; human.;
-