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Alternative name
Vacuolar protein sorting-associated protein 13D ELISA KIT; VPS13D ELISA KIT;
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Catalog
E072284
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species
Mouse
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GeneVPS13D
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Vacuolar Protein Sorting 13D (VPS13D).
No significant cross-reactivity or interference between Vacuolar Protein Sorting 13D (VPS13D) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.057ng/mL.
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Intended UseMouse VPS13D ELISA Kit allows for the in vitro quantitative determination of VPS13D , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vacuolar Protein Sorting 13D (VPS13D). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vacuolar Protein Sorting 13D (VPS13D). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vacuolar Protein Sorting 13D (VPS13D), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vacuolar Protein Sorting 13D (VPS13D) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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