Mouse Vldlr ELISA Kit

Alternative name
Mouse VLDL receptor ELISA Kit;Mouse VLDL-R ELISA Kit;Mouse AA408956 ELISA Kit;Mouse AI451093 ELISA Kit;Mouse AW047288 ELISA Kit;Mouse very low density lipoprotein receptor ELISA Kit;Mouse very low-density lipoprotein receptor ELISA Kit;
Catalog
E072464
species
Mouse
GeneVldlr
SpecificityThis assay has high sensitivity and excellent specificity for detection of VLDLR. No significant cross-reactivity or interference between VLDLR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between VLDLR and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Vldlr ELISA Kit allows for the in vitro quantitative determination of Vldlr , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: VLDLR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-VLDLR antibody and an VLDLR-HRP conjugate. The assay sample and buffer are incubated together with VLDLR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VLDLR concentration since VLDLR from samples and VLDLR-HRP conjugate compete for the anti-VLDLR antibody binding site. Since the number of sites is limited, as more sites are occupied by VLDLR from the sample, fewer sites are left to bind VLDLR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VLDLR concentration in each sample is interpolated from this standard curve.
Mouse Very low-density lipoprotein receptor Protein information
Uniprot ID VLDLR_MOUSE
Uniprot AC P98156; Q64022;
UniGene Mm.4141;
GeneID 22359
KEGG mmu:22359;
Mouse Very low-density lipoprotein receptor Protein SEQUENCE
SEQUENCE 873 AA; 96373 MW; 08F09F93825195CB CRC64;
MGTSARWALW LLLALCWAPR DSGATASGKK AKCDSSQFQC TNGRCITLLW
KCDGDEDCAD GSDEKNCVKK TCAESDFVCK NGQCVPNRWQ CDGDPDCEDG
SDESPEQCHM RTCRINEISC GARSTQCIPV SWRCDGENDC DNGEDEENCG
NITCSADEFT CSSGRCVSRN FVCNGQDDCD DGSDELDCAP PTCGAHEFQC
STSSCIPLSW VCDDDADCSD QSDESLEQCG RQPVIHTKCP TSEIQCGSGE
CIHKKWRCDG DPDCKDGSDE VNCPSRTCRP DQFECEDGSC IHGSRQCNGI
RDCVDGSDEV NCKNVNQCLG PGKFKCRSGE CIDMSKVCDQ EQDCRDWSDE
PLKECHINEC LVNNGGCSHI CKDLVIGYEC DCAAGFELID RKTCGDIDEC
QNPGICSQIC INLKGGYKCE CSRGYQMDLA TGVCKAVGKE PSLIFTNRRD
IRKIGLERKE YIQLVEQLRN TVALDADIAA QKLFWADLSQ KAIFSASIDD
KVGRHFKMID NVYNPAAIAV DWVYKTIYWT DAASKTISVA TLDGAKRKFL
FNSDLREPAS IAVDPLSGFV YWSDWGEPAK IEKAGMNGFD RRPLVTEDIQ
WPNGITLDLV KSRLYWLDSK LHMLSSVDLN GQDRRIVLKS LEFLAHPLAL
TIFEDRVYWI DGENEAVYGA NKFTGSELAT LVNNLNDAQD IIVYHELVQP
SGKNWCEDDM ENGGCEYLCL PAPQINDHSP KYTCSCPNGY NLEENGRECQ
STSTPVTYSE TKDINTTDIL RTSGLVPGGI NVTTAVSEVS VPPKGTSAAW
AILPLLLLVM AAVGGYLMWR NWQHKNMKSM NFDNPVYLKT TEEDLSIDIG
RHSASVGHTY PAISVVSTDD DLA
UCSC uc008hbt.2; mouse.;

Category

Mouse Vldlr ELISA Kit

Mouse VLDL receptor ELISA Kit;Mouse VLDL-R ELISA Kit;Mouse AA408956 ELISA Kit;Mouse AI451093 ELISA Kit;Mouse AW047288 ELISA Kit;Mouse very low density lipoprotein receptor ELISA Kit;Mouse very low-density lipoprotein receptor ELISA Kit;