Mouse Vim ELISA Kit

Alternative name
Mouse vimentin ELISA Kit;
Catalog
E072504
species
Mouse
GeneVim
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse VIM. No significant cross-reactivity or interference between Mouse VIM and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.12 pg/ml
Intended UseMouse Vim ELISA Kit allows for the in vitro quantitative determination of Vim , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VIM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Vimentin Protein information
Uniprot ID VIME_MOUSE
Uniprot AC P20152; O08704; Q8CCH1;
UniGene Mm.268000;
GeneID 22352
KEGG mmu:22352;
Mouse Vimentin Protein SEQUENCE
SEQUENCE 466 AA; 53688 MW; A94EECEA6D70C899 CRC64;
MSTRSVSSSS YRRMFGGSGT SSRPSSNRSY VTTSTRTYSL GSALRPSTSR
SLYSSSPGGA YVTRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR
TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY
EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE
STLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHDE EIQELQAQIQ
EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE
AANRNNDALR QAKQESNEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN
FALEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY
RKLLEGEESR ISLPLPTFSS LNLRETNLES LPLVDTHSKR TLLIKTVETR
DGQVINETSQ HHDDLE
UCSC uc008ikb.2; mouse.;

Category

Mouse Vim ELISA Kit

Mouse vimentin ELISA Kit;