-
Alternative name
CD40 ligand ELISA KIT; T-cell antigen Gp39 ELISA KIT; TNF-related activation protein ELISA KIT; TRAP ELISA KIT; Tumor necrosis factor ligand superfamily member 5 ELISA KIT; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form ELISA KIT; CD40 ligand, soluble formCD40LG ELISA KIT; CD40L ELISA KIT; TNFSF5 ELISA KIT; TRAP ELISA KIT; CD40-L ELISA KIT; TRAP ELISA KIT
-
Catalog
E007256
-
species
Human
-
GeneCD40L
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
Sensitivity0.1 ng/mL.
-
Intended UseHuman CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Categories/FamilyImmunology
-
Product Description
specificalPrinciple of the Assay: CD40L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD40L antibody and an CD40L-HRP conjugate. The assay sample and buffer are incubated together with CD40L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD40L concentration since CD40L from samples and CD40L-HRP conjugate compete for the anti-CD40L antibody binding site. Since the number of sites is limited, as more sites are occupied by CD40L from the sample, fewer sites are left to bind CD40L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD40L concentration in each sample is interpolated from this standard curve.
-