Mouse Wnt4 ELISA Kit

Alternative name
Mouse Wnt-4 ELISA Kit;Mouse wingless-type MMTV integration site family, member 4 ELISA Kit;Mouse protein Wnt-4 ELISA Kit;Mouse signal molecule ELISA Kit;Mouse wingless-related MMTV integration site 4 ELISA Kit;
Catalog
E072778
species
Mouse
GeneWnt4
SpecificityThis assay has high sensitivity and excellent specificity for detection of WNT4. No significant cross-reactivity or interference between WNT4 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of WNT4 is typically less than 0.054ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseMouse Wnt4 ELISA Kit allows for the in vitro quantitative determination of Wnt4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Protein Wnt-4 Protein information
Uniprot ID WNT4_MOUSE
Uniprot AC P22724;
UniGene Mm.20355;
GeneID 22417
KEGG mmu:22417;
Mouse Protein Wnt-4 Protein SEQUENCE
SEQUENCE 351 AA; 39050 MW; 7E1C5C739BE939D9 CRC64;
MSPRSCLRSL RLLVFAVFSA AASNWLYLAK LSSVGSISEE ETCEKLKGLI
QRQVQMCKRN LEVMDSVRRG AQLAIEECQY QFRNRRWNCS TLDSLPVFGK
VVTQGTREAA FVYAISSAGV AFAVTRACSS GELEKCGCDR TVHGVSPQGF
QWSGCSDNIA YGVAFSQSFV DVRERSKGAS SSRALMNLHN NEAGRKAILT
HMRVECKCHG VSGSCEVKTC WRAVPPFRQV GHALKEKFDG ATEVEPRRVG
SSRALVPRNA QFKPHTDEDL VYLEPSPDFC EQDIRSGVLG TRGRTCNKTS
KAIDGCELLC CGRGFHTAQV ELAERCGCRF HWCCFVKCRQ CQRLVEMHTC
R
UCSC uc008viv.2; mouse.;

Category

Mouse Wnt4 ELISA Kit

Mouse Wnt-4 ELISA Kit;Mouse wingless-type MMTV integration site family, member 4 ELISA Kit;Mouse protein Wnt-4 ELISA Kit;Mouse signal molecule ELISA Kit;Mouse wingless-related MMTV integration site 4 ELISA Kit;