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Human SIGLEC1 ELISA Kit

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  • Alternative name

    Human Sialic acid-binding Ig-like lectin 1 ELISA Kit;Human SIGLEC-1 ELISA Kit;Human SN ELISA Kit;Human CD169 ELISA Kit;Human dJ1009E24.1 ELISA Kit;Human sialic acid binding Ig like lectin 1 ELISA Kit;Human sialoadhesin ELISA Kit;Human sialic acid binding Ig-like lectin 1, sialoadhesin ELISA Kit;Human sialic acid-binding immunoglobulin-like lectin 1 ELISA Kit;
  • Catalog
    E007323
  • species
    Human
  • GeneSIGLEC1
  • SpecificityThe Human CD169 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD169 proteins within the range of 156 pg/ml - 10000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman SIGLEC1 ELISA Kit allows for the in vitro quantitative determination of SIGLEC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Human CD169 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD169 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD169 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD169 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD169 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD169 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: SIGLEC-1, also known as Sialoadhesin or CD169, is a cell adhesion molecule found on the surface of certain cells of the immune system called macrophages. This gene is mapped to 20p13. It belongs to the immunoglobulin superfamily(IgSF). Since sialoadhesin binds sialic acids with its N-terminal IgV-domain, it is also a member of the SIGLEC family. The localization and expression of SIGLEC-1 in humans has altered coincident with the evolutionary loss of Neu5Gc. SIGLEC-1-positive macrophages have a dual physiologic function. They act as innate 'flypaper' by preventing the systemic spread of lymph-borne pathogens and as critical gatekeepers at the lymph-tissue interface that facilitate the recognition of particulate antigens by B cells and initiate humoral immune responses.
  • Human Sialoadhesin Protein information
  • Uniprot ID SN_HUMAN
  • Uniprot AC Q9BZZ2; Q96DL4; Q9GZS5; Q9H1H6; Q9H1H7; Q9H7L7;
  • UniGene Hs.31869;
  • GeneID 6614
  • KEGG hsa:6614;
  • Human Sialoadhesin Protein SEQUENCE

  • SEQUENCE 1709 AA; 182624 MW; 587C7CCA0B789A6D CRC64;

    MGFLPKLLLL ASFFPAGQAS WGVSSPQDVQ GVKGSCLLIP CIFSFPADVE

    VPDGITAIWY YDYSGQRQVV SHSADPKLVE ARFRGRTEFM GNPEHRVCNL

    LLKDLQPEDS GSYNFRFEIS EVNRWSDVKG TLVTVTEEPR VPTIASPVEL

    LEGTEVDFNC STPYVCLQEQ VRLQWQGQDP ARSVTFNSQK FEPTGVGHLE

    TLHMAMSWQD HGRILRCQLS VANHRAQSEI HLQVKYAPKG VKILLSPSGR

    NILPGELVTL TCQVNSSYPA VSSIKWLKDG VRLQTKTGVL HLPQAAWSDA

    GVYTCQAENG VGSLVSPPIS LHIFMAEVQV SPAGPILENQ TVTLVCNTPN

    EAPSDLRYSW YKNHVLLEDA HSHTLRLHLA TRADTGFYFC EVQNVHGSER

    SGPVSVVVNH PPLTPVLTAF LETQAGLVGI LHCSVVSEPL ATLVLSHGGH

    ILASTSGDSD HSPRFSGTSG PNSLRLEIRD LEETDSGEYK CSATNSLGNA

    TSTLDFHANA ARLLISPAAE VVEGQAVTLS CRSGLSPTPD ARFSWYLNGA

    LLHEGPGSSL LLPAASSTDA GSYHCRARDG HSASGPSSPA VLTVLYPPRQ

    PTFTTRLDLD AAGAGAGRRG LLLCRVDSDP PARLQLLHKD RVVATSLPSG

    GGCSTCGGCS PRMKVTKAPN LLRVEIHNPL LEEEGLYLCE ASNALGNAST

    SATFNGQATV LAIAPSHTLQ EGTEANLTCN VSREAAGSPA NFSWFRNGVL

    WAQGPLETVT LLPVARTDAA LYACRILTEA GAQLSTPVLL SVLYPPDRPK

    LSALLDMGQG HMALFICTVD SRPLALLALF HGEHLLATSL GPQVPSHGRF

    QAKAEANSLK LEVRELGLGD SGSYRCEATN VLGSSNTSLF FQVRGAWVQV

    SPSPELQEGQ AVVLSCQVHT GVPEGTSYRW YRDGQPLQES TSATLRFAAI

    TLTQAGAYHC QAQAPGSATT SLAAPISLHV SYAPRHVTLT TLMDTGPGRL

    GLLLCRVDSD PPAQLRLLHG DRLVASTLQG VGGPEGSSPR LHVAVAPNTL

    RLEIHGAMLE DEGVYICEAS NTLGQASASA DFDAQAVNVQ VWPGATVREG

    QLVNLTCLVW TTHPAQLTYT WYQDGQQRLD AHSIPLPNVT VRDATSYRCG

    VGPPGRAPRL SRPITLDVLY APRNLRLTYL LESHGGQLAL VLCTVDSRPP

    AQLALSHAGR LLASSTAASV PNTLRLELRG PQPRDEGFYS CSARSPLGQA

    NTSLELRLEG VRVILAPEAA VPEGAPITVT CADPAAHAPT LYTWYHNGRW

    LQEGPAASLS FLVATRAHAG AYSCQAQDAQ GTRSSRPAAL QVLYAPQDAV

    LSSFRDSRAR SMAVIQCTVD SEPPAELALS HDGKVLATSS GVHSLASGTG

    HVQVARNALR LQVQDVPAGD DTYVCTAQNL LGSISTIGRL QVEGARVVAE

    PGLDVPEGAA LNLSCRLLGG PGPVGNSTFA WFWNDRRLHA EPVPTLAFTH

    VARAQAGMYH CLAELPTGAA ASAPVMLRVL YPPKTPTMMV FVEPEGGLRG

    ILDCRVDSEP LASLTLHLGS RLVASSQPQG APAEPHIHVL ASPNALRVDI

    EALRPSDQGE YICSASNVLG SASTSTYFGV RALHRLHQFQ QLLWVLGLLV

    GLLLLLLGLG ACYTWRRRRV CKQSMGENSV EMAFQKETTQ LIDPDAATCE

    TSTCAPPLG

  • UCSC uc002wja.3; human. [Q9BZZ2-1];



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