Human CD177 ELISA Kit

Alternative name
Human human neutrophil alloantigen 2a ELISA Kit;Human HNA-2a ELISA Kit;Human NB1 glycoprotein ELISA Kit;Human NB1 GP ELISA Kit;Human polycythemia rubra vera protein 1 ELISA Kit;Human PRV-1 ELISA Kit;Human NB1 ELISA Kit;Human PRV1 ELISA Kit;Human HNA2A ELISA Kit;Human CD177 molecule ELISA Kit;Human CD177 antigen ELISA Kit;Human cell surface receptor ELISA Kit;
Catalog
E007327
species
Human
GeneCD177
SpecificityThis assay has high sensitivity and excellent specificity for detection of CD177. No significant cross-reactivity or interference between CD177 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CD177 ELISA Kit allows for the in vitro quantitative determination of CD177 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the assay: CD177 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD177 antibody and an CD177-HRP conjugate. The assay sample and buffer are incubated together with CD177-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD177 concentration since CD177 from samples and CD177-HRP conjugate compete for the anti-CD177 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD177 from the sample, fewer sites are left to bind CD177-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD177 concentration in each sample is interpolated from this standard curve.
Human CD177 antigen Protein information
Uniprot ID CD177_HUMAN
Uniprot AC Q8N6Q3; Q711Q2; Q8NCV9; Q96QH1; Q9HDA5;
UniGene Hs.232165;
GeneID 57126
KEGG hsa:57126;
Human CD177 antigen Protein SEQUENCE
SEQUENCE 437 AA; 46363 MW; 3D4716F221934910 CRC64;
MSAVLLLALL GFILPLPGVQ ALLCQFGTVQ HVWKVSDLPR QWTPKNTSCD
SGLGCQDTLM LIESGPQVSL VLSKGCTEAK DQEPRVTEHR MGPGLSLISY
TFVCRQEDFC NNLVNSLPLW APQPPADPGS LRCPVCLSME GCLEGTTEEI
CPKGTTHCYD GLLRLRGGGI FSNLRVQGCM PQPGCNLLNG TQEIGPVGMT
ENCNRKDFLT CHRGTTIMTH GNLAQEPTDW TTSNTEMCEV GQVCQETLLL
LDVGLTSTLV GTKGCSTVGA QNSQKTTIHS APPGVLVASY THFCSSDLCN
SASSSSVLLN SLPPQAAPVP GDRQCPTCVQ PLGTCSSGSP RMTCPRGATH
CYDGYIHLSG GGLSTKMSIQ GCVAQPSSFL LNHTRQIGIF SAREKRDVQP
PASQHEGGGA EGLESLTWGV GLALAPALWW GVVCPSC
UCSC uc060zla.1; human. [Q8N6Q3-1];

Category

Human CD177 ELISA Kit