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Catalog
E073469
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species
Mouse
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Gene15-S-HETE
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse 15-S-HETE ELISA Kit allows for the in vitro quantitative determination of 15-S-HETE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCell Biology
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Product Description
specificalPrinciple of the Assay: 15-S-HETE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-15-S-HETE antibody and an 15-S-HETE-HRP conjugate. The assay sample and buffer are incubated together with 15-S-HETE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 15-S-HETE concentration since 15-S-HETE from samples and 15-S-HETE-HRP conjugate compete for the anti-15-S-HETE antibody binding site. Since the number of sites is limited, as more sites are occupied by 15-S-HETE from the sample, fewer sites are left to bind 15-S-HETE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 15-S-HETE concentration in each sample is interpolated from this standard curve.
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