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Mouse Hsd17b10 ELISA Kit

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  • Alternative name

    Mouse 17-beta-hydroxysteroid dehydrogenase 10 ELISA Kit;Mouse 17-beta-HSD 10 ELISA Kit;Mouse 3-hydroxy-2-methylbutyryl-CoA dehydrogenase ELISA Kit;Mouse 3-hydroxyacyl-CoA dehydrogenase type II ELISA Kit;Mouse Endoplasmic reticulum-associated amyloid beta-peptide-binding protein ELISA Kit;Mouse Mitochondrial ribonuclease P protein 2 ELISA Kit;Mouse Mitochondrial RNase P protein 2 ELISA Kit;Mouse Type II HADH ELISA Kit;Mouse Erab ELISA Kit;Mouse Hadh2 ELISA Kit;
  • Catalog
    E073495
  • species
    Mouse
  • GeneHsd17b10
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). No significant cross-reactivity or interference between 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.063ng/mL.
  • Intended UseMouse Hsd17b10 ELISA Kit allows for the in vitro quantitative determination of Hsd17b10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse 3-hydroxyacyl-CoA dehydrogenase type-2 Protein information
  • Uniprot ID HCD2_MOUSE
  • Uniprot AC O08756;
  • UniGene Mm.6994;
  • Mouse 3-hydroxyacyl-CoA dehydrogenase type-2 Protein SEQUENCE

  • SEQUENCE 261 AA; 27419 MW; 61213B13E2839D41 CRC64;

    MAAAVRSVKG LVAVVTGGAS GPWLATAKRL VGQGATAVLL DVPDSEGESQ

    AKKLGESCIF APANVTSEKE IQAALTLAKE KFGRIDVAVN CAGIAVAIKT

    YHQKKNKIHT LEDFQRVINV NLIGTFNVIR LVAGEMGQNE PDQGGQRGVI

    INTASVAAFE GQVGQAAYSA SKGGIDGMTL PIARDLAPTG IRVVTIAPGL

    FATPLLTTLP EKVRNFLASQ VPFPSRLGDP AEYAHLVQTI IENPFLNGEV

    IRLDGAIRMQ P




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