Human CD276 ELISA Kit

Alternative name
Human 4Ig-B7-H3 ELISA Kit;Human B7 homolog 3 ELISA Kit;Human B7-H3 ELISA Kit;Human costimulatory molecule ELISA Kit;Human B7H3 ELISA Kit;Human B7RP-2 ELISA Kit;Human CD276 molecule ELISA Kit;Human CD276 antigen ELISA Kit;
Catalog
E007404
species
Human
GeneCD276
SpecificityThis assay has high sensitivity and excellent specificity for detection of human sCD276/sB7-H3. No significant cross-reactivity or interference between human sCD276/sB7-H3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human sCD276/sB7-H3 is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined
Intended UseHuman CD276 ELISA Kit allows for the in vitro quantitative determination of CD276 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD276/sB7-H3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD276/sB7-H3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD276/sB7-H3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD276/sB7-H3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human CD276 antigen Protein information
Uniprot ID CD276_HUMAN
Uniprot AC Q5ZPR3; Q6P5Y4; Q6UXI2; Q8NBI8; Q8NC34; Q8NCB6; Q9BXR1;
UniGene Hs.744915;
GeneID 80381
KEGG hsa:80381;
Human CD276 antigen Protein SEQUENCE
SEQUENCE 534 AA; 57235 MW; 2181A064404C7939 CRC64;
MLRRRGSPGM GVHVGAALGA LWFCLTGALE VQVPEDPVVA LVGTDATLCC
SFSPEPGFSL AQLNLIWQLT DTKQLVHSFA EGQDQGSAYA NRTALFPDLL
AQGNASLRLQ RVRVADEGSF TCFVSIRDFG SAAVSLQVAA PYSKPSMTLE
PNKDLRPGDT VTITCSSYQG YPEAEVFWQD GQGVPLTGNV TTSQMANEQG
LFDVHSILRV VLGANGTYSC LVRNPVLQQD AHSSVTITPQ RSPTGAVEVQ
VPEDPVVALV GTDATLRCSF SPEPGFSLAQ LNLIWQLTDT KQLVHSFTEG
RDQGSAYANR TALFPDLLAQ GNASLRLQRV RVADEGSFTC FVSIRDFGSA
AVSLQVAAPY SKPSMTLEPN KDLRPGDTVT ITCSSYRGYP EAEVFWQDGQ
GVPLTGNVTT SQMANEQGLF DVHSVLRVVL GANGTYSCLV RNPVLQQDAH
GSVTITGQPM TFPPEALWVT VGLSVCLIAL LVALAFVCWR KIKQSCEEEN
AGAEDQDGEG EGSKTALQPL KHSDSKEDDG QEIA
UCSC uc002avu.2; human. [Q5ZPR3-1];

Category

Human CD276 ELISA Kit

Human 4Ig-B7-H3 ELISA Kit;Human B7 homolog 3 ELISA Kit;Human B7-H3 ELISA Kit;Human costimulatory molecule ELISA Kit;Human B7H3 ELISA Kit;Human B7RP-2 ELISA Kit;Human CD276 molecule ELISA Kit;Human CD276 antigen ELISA Kit;