Human NRP1 ELISA Kit

Alternative name
Human vascular endothelial cell growth factor 165 receptor ELISA Kit;Human NRP ELISA Kit;Human VEGF165R ELISA Kit;Human BDCA4 ELISA Kit;Human CD304 ELISA Kit;Human NP1 ELISA Kit;Human neuropilin 1 ELISA Kit;Human neuropilin-1 ELISA Kit;Human transmembrane receptor ELISA Kit;
Catalog
E007423
species
Human
GeneNRP1
SpecificityThe Human CD304 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD304 proteins within the range of 780 pg/ml - 50000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity20 pg/ml.
Intended UseHuman NRP1 ELISA Kit allows for the in vitro quantitative determination of NRP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human CD304 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD304 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD304 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD304 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD304 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD304 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: NRP1 (Neuropilin 1) also known as NP1, NRP, BDCA4 or VEGF165R, is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF) and semaphorin family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion. By somatic cell hybrid analysis, the NRP1 gene was mapped to chromosome 10. NRP1 bounds PGF1 with lower affinity. NRP1-mediated interactions are a necessary element in the initiation of the primary immune response and offer another example, like that of agrin, of a molecule shared by neurologic and immunologic synapses. After T-cell contact with DC, T-cell NRP1 colocalized with CD3 in the immunologic synapse and, sometimes, also at the opposite pole of the T cell. Soluble NRP1 interacts in a homophilic fashion with NRP1 on both DC and T cells, and this binding can be inhibited by blocking antibodies to NRP1. Furthermore, selective NRP1 inhibition in this model suppressed neovascular formation substantially.
Human Neuropilin-1 Protein information
Uniprot ID NRP1_HUMAN
Uniprot AC Q96IH5;
UniGene Hs.131704; Hs.653996;
GeneID 8829
KEGG hsa:8829;
Human Neuropilin-1 Protein SEQUENCE
SEQUENCE 923 AA; 103134 MW; 1EAC2FA6C8FD6A0B CRC64;
MERGLPLLCA VLALVLAPAG AFRNDKCGDT IKIESPGYLT SPGYPHSYHP
SEKCEWLIQA PDPYQRIMIN FNPHFDLEDR DCKYDYVEVF DGENENGHFR
GKFCGKIAPP PVVSSGPFLF IKFVSDYETH GAGFSIRYEI FKRGPECSQN
YTTPSGVIKS PGFPEKYPNS LECTYIVFVP KMSEIILEFE SFDLEPDSNP
PGGMFCRYDR LEIWDGFPDV GPHIGRYCGQ KTPGRIRSSS GILSMVFYTD
SAIAKEGFSA NYSVLQSSVS EDFKCMEALG MESGEIHSDQ ITASSQYSTN
WSAERSRLNY PENGWTPGED SYREWIQVDL GLLRFVTAVG TQGAISKETK
KKYYVKTYKI DVSSNGEDWI TIKEGNKPVL FQGNTNPTDV VVAVFPKPLI
TRFVRIKPAT WETGISMRFE VYGCKITDYP CSGMLGMVSG LISDSQITSS
NQGDRNWMPE NIRLVTSRSG WALPPAPHSY INEWLQIDLG EEKIVRGIII
QGGKHRENKV FMRKFKIGYS NNGSDWKMIM DDSKRKAKSF EGNNNYDTPE
LRTFPALSTR FIRIYPERAT HGGLGLRMEL LGCEVEAPTA GPTTPNGNLV
DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTVIDSTIQ SEFPTYGFNC
EFGWGSHKTF CHWEHDNHVQ LKWSVLTSKT GPIQDHTGDG NFIYSQADEN
QKGKVARLVS PVVYSQNSAH CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ
LVWMAIGHQG DHWKEGRVLL HKSLKLYQVI FEGEIGKGNL GGIAVDDISI
NNHISQEDCA KPADLDKKNP EIKIDETGST PGYEGEGEGD KNISRKPGNV
LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN
FELVDGVKLK KDKLNTQSTY SEA
UCSC uc001iwx.5; human. [O14786-1];

Category

Human NRP1 ELISA Kit

Human vascular endothelial cell growth factor 165 receptor ELISA Kit;Human NRP ELISA Kit;Human VEGF165R ELISA Kit;Human BDCA4 ELISA Kit;Human CD304 ELISA Kit;Human NP1 ELISA Kit;Human neuropilin 1 ELISA Kit;Human neuropilin-1 ELISA Kit;Human transmembrane receptor ELISA Kit;