Human SELE ELISA Kit

Alternative name
Human CD62 antigen-like family member E ELISA Kit;Human endothelial leukocyte adhesion molecule 1 ELISA Kit;Human ELAM-1 ELISA Kit;Human Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit;Human LECAM2 ELISA Kit;Human ELAM1 ELISA Kit;Human CD62E ELISA Kit;Human ELAM ELISA Kit;Human ESEL ELISA Kit;Human selectin E ELISA Kit;Human E-selectin ELISA Kit;Human endothelial adhesion molecule 1 ELISA Kit;Human leukocyte endothelial cell adhesion molecule 2 ELISA Kit;
Catalog
E007506
species
Human
GeneSELE
SpecificityThe Human CD62E ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD62E proteins within the range of 781.3 pg/ml - 50000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity200 pg/ml.
Intended UseHuman SELE ELISA Kit allows for the in vitro quantitative determination of SELE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human CD62E ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62E in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD62E coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.
Human E-selectin Protein information
Uniprot ID LYAM2_HUMAN
Uniprot AC P16581; A2RRD6; P16111;
UniGene Hs.82848;
GeneID 6401
KEGG hsa:6401;
Human E-selectin Protein SEQUENCE
SEQUENCE 610 AA; 66655 MW; 7D43E3C0D1229229 CRC64;
MIASQFLSAL TLVLLIKESG AWSYNTSTEA MTYDEASAYC QQRYTHLVAI
QNKEEIEYLN SILSYSPSYY WIGIRKVNNV WVWVGTQKPL TEEAKNWAPG
EPNNRQKDED CVEIYIKREK DVGMWNDERC SKKKLALCYT AACTNTSCSG
HGECVETINN YTCKCDPGFS GLKCEQIVNC TALESPEHGS LVCSHPLGNF
SYNSSCSISC DRGYLPSSME TMQCMSSGEW SAPIPACNVV ECDAVTNPAN
GFVECFQNPG SFPWNTTCTF DCEEGFELMG AQSLQCTSSG NWDNEKPTCK
AVTCRAVRQP QNGSVRCSHS PAGEFTFKSS CNFTCEEGFM LQGPAQVECT
TQGQWTQQIP VCEAFQCTAL SNPERGYMNC LPSASGSFRY GSSCEFSCEQ
GFVLKGSKRL QCGPTGEWDN EKPTCEAVRC DAVHQPPKGL VRCAHSPIGE
FTYKSSCAFS CEEGFELHGS TQLECTSQGQ WTEEVPSCQV VKCSSLAVPG
KINMSCSGEP VFGTVCKFAC PEGWTLNGSA ARTCGATGHW SGLLPTCEAP
TESNIPLVAG LSAAGLSLLT LAPFLLWLRK CLRKAKKFVP ASSCQSLESD
GSYQKPSYIL
UCSC uc001ggm.5; human.;

Category

Human SELE ELISA Kit