Human SELP ELISA Kit

Alternative name
Human CD62 antigen-like family member P ELISA Kit;Human granule membrane protein 140 ELISA Kit;Human GMP-140 ELISA Kit;Human leukocyte-endothelial cell adhesion molecule 3 ELISA Kit;Human LECAM3 ELISA Kit;Human platelet activation dependent granule-external membrane protein ELISA Kit;Human PADGEM ELISA Kit;Human GMRP ELISA Kit;Human GRMP ELISA Kit;Human CD62 ELISA Kit;Human CD62P ELISA Kit;Human GMP140 ELISA Kit;Human PSEL ELISA Kit;Human selectin P ELISA Kit;Human P-selectin ELISA Kit;Human granule membrane protein 140kDa ELISA Kit;Human granulocyte membrane protein ELISA Kit;Human platelet alpha-granule membrane protein ELISA Kit;Human selectin P (granule membrane protein 140kDa, antigen CD62) ELISA Kit;
Catalog
E007511
species
Human
GeneSELP
SpecificityThe Human CD62P ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD62P proteins within the range of 125 pg/ml - 8000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity30 pg/ml.
Intended UseHuman SELP ELISA Kit allows for the in vitro quantitative determination of SELP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human CD62P ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD62P in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD62P coated on a 96-well plate. Standards and samples are pipetted into the wells and CD62P present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD62P antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD62P bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1).
Human P-selectin Protein information
Uniprot ID LYAM3_HUMAN
Uniprot AC P16109; Q5R344; Q8IVD1;
UniGene Hs.73800;
GeneID 6403
KEGG hsa:6403;
Human P-selectin Protein SEQUENCE
SEQUENCE 830 AA; 90834 MW; F53BC476AB6F70AC CRC64;
MANCQIAILY QRFQRVVFGI SQLLCFSALI SELTNQKEVA AWTYHYSTKA
YSWNISRKYC QNRYTDLVAI QNKNEIDYLN KVLPYYSSYY WIGIRKNNKT
WTWVGTKKAL TNEAENWADN EPNNKRNNED CVEIYIKSPS APGKWNDEHC
LKKKHALCYT ASCQDMSCSK QGECLETIGN YTCSCYPGFY GPECEYVREC
GELELPQHVL MNCSHPLGNF SFNSQCSFHC TDGYQVNGPS KLECLASGIW
TNKPPQCLAA QCPPLKIPER GNMTCLHSAK AFQHQSSCSF SCEEGFALVG
PEVVQCTASG VWTAPAPVCK AVQCQHLEAP SEGTMDCVHP LTAFAYGSSC
KFECQPGYRV RGLDMLRCID SGHWSAPLPT CEAISCEPLE SPVHGSMDCS
PSLRAFQYDT NCSFRCAEGF MLRGADIVRC DNLGQWTAPA PVCQALQCQD
LPVPNEARVN CSHPFGAFRY QSVCSFTCNE GLLLVGASVL QCLATGNWNS
VPPECQAIPC TPLLSPQNGT MTCVQPLGSS SYKSTCQFIC DEGYSLSGPE
RLDCTRSGRW TDSPPMCEAI KCPELFAPEQ GSLDCSDTRG EFNVGSTCHF
SCDNGFKLEG PNNVECTTSG RWSATPPTCK GIASLPTPGL QCPALTTPGQ
GTMYCRHHPG TFGFNTTCYF GCNAGFTLIG DSTLSCRPSG QWTAVTPACR
AVKCSELHVN KPIAMNCSNL WGNFSYGSIC SFHCLEGQLL NGSAQTACQE
NGHWSTTVPT CQAGPLTIQE ALTYFGGAVA STIGLIMGGT LLALLRKRFR
QKDDGKCPLN PHSHLGTYGV FTNAAFDPSP
UCSC uc001ggi.5; human.;

Category

Human SELP ELISA Kit