Human NT5E ELISA Kit

Alternative name
Human 5'-NT ELISA Kit;Human ecto-5'-nucleotidase ELISA Kit;Human NT5 ELISA Kit;Human NTE ELISA Kit;Human CALJA ELISA Kit;Human CD73 ELISA Kit;Human E5NT ELISA Kit;Human NT ELISA Kit;Human eN ELISA Kit;Human eNT ELISA Kit;Human 5'-nucleotidase ecto ELISA Kit;Human 5'-nucleotidase ELISA Kit;Human Purine 5-Prime-Nucleotidase ELISA Kit;
Catalog
E007525
species
Human
GeneNT5E
SpecificityNatural and recombinant human CD73
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human CD73 in cell culture supernates, serum and plasm
Intended UseHuman NT5E ELISA Kit allows for the in vitro quantitative determination of NT5E , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human CD73 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD73 has been precoated onto 96-well plates. Standards(CHO, W27-K547) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD73 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD73 amount of sample captured in plate. Background: 5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Human 5'-nucleotidase Protein information
Uniprot ID 5NTD_HUMAN
Uniprot AC P21589; B3KQI8; O75520; Q5W116;
UniGene Hs.153952;
GeneID 4907
KEGG hsa:4907;
Human 5'-nucleotidase Protein SEQUENCE
SEQUENCE 574 AA; 63368 MW; A99AF170AB7EAECE CRC64;
MCPRAARAPA TLLLALGAVL WPAAGAWELT ILHTNDVHSR LEQTSEDSSK
CVNASRCMGG VARLFTKVQQ IRRAEPNVLL LDAGDQYQGT IWFTVYKGAE
VAHFMNALRY DAMALGNHEF DNGVEGLIEP LLKEAKFPIL SANIKAKGPL
ASQISGLYLP YKVLPVGDEV VGIVGYTSKE TPFLSNPGTN LVFEDEITAL
QPEVDKLKTL NVNKIIALGH SGFEMDKLIA QKVRGVDVVV GGHSNTFLYT
GNPPSKEVPA GKYPFIVTSD DGRKVPVVQA YAFGKYLGYL KIEFDERGNV
ISSHGNPILL NSSIPEDPSI KADINKWRIK LDNYSTQELG KTIVYLDGSS
QSCRFRECNM GNLICDAMIN NNLRHTDEMF WNHVSMCILN GGGIRSPIDE
RNNGTITWEN LAAVLPFGGT FDLVQLKGST LKKAFEHSVH RYGQSTGEFL
QVGGIHVVYD LSRKPGDRVV KLDVLCTKCR VPSYDPLKMD EVYKVILPNF
LANGGDGFQM IKDELLRHDS GDQDINVVST YISKMKVIYP AVEGRIKFST
GSHCHGSFSL IFLSLWAVIF VLYQ
UCSC uc003pko.5; human. [P21589-1];

Category

Human NT5E ELISA Kit