Human FAS ELISA Kit

Alternative name
Human Apo-1 antigen ELISA Kit;Human apoptosis-mediating surface antigen FAS ELISA Kit;Human FASLG receptor ELISA Kit;Human APT1 ELISA Kit;Human FAS1 ELISA Kit;Human TNFRSF6 ELISA Kit;Human ALPS1A ELISA Kit;Human APO-1 ELISA Kit;Human CD95 ELISA Kit;Human FASTM ELISA Kit;Human Fas cell surface death receptor ELISA Kit;Human tumor necrosis factor receptor superfamily member 6 ELISA Kit;Human APO-1 cell surface antigen ELISA Kit;Human CD95 antigen ELISA Kit;Human Fas (TNF receptor superfamily, member 6) ELISA Kit;Human Fas AMA ELISA Kit;Human TNF receptor superfamily member 6 ELISA Kit;Human apoptosis antigen 1 ELISA Kit;Human apoptosis signaling receptor FAS ELISA Kit;Human mutant tumor necrosis receptor superfamily member 6 ELISA Kit;Human tumor necrosis factor receptor superfamily, member 6 ELISA Kit;
Catalog
E007550
species
Human
GeneFAS
SpecificityThe Human CD95 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human CD95 proteins within the range of 125 pg/ml - 8000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity62 pg/ml.
Intended UseHuman FAS ELISA Kit allows for the in vitro quantitative determination of FAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||The Human CD95 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD95 in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human CD95 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD95 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD95 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD95 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis. The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40. The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48 kDa. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo. The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.
Human Tumor necrosis factor receptor superfamily member 6 Protein information
Uniprot ID TNR6_HUMAN
Uniprot AC Q5T9P1; Q5T9P2; Q5T9P3; Q6SSE9;
UniGene Hs.244139; Hs.667309;
GeneID 355
KEGG hsa:355;
Human Tumor necrosis factor receptor superfamily member 6 Protein SEQUENCE
SEQUENCE 335 AA; 37732 MW; 0139942535111410 CRC64;
MLGIWTLLPL VLTSVARLSS KSVNAQVTDI NSKGLELRKT VTTVETQNLE
GLHHDGQFCH KPCPPGERKA RDCTVNGDEP DCVPCQEGKE YTDKAHFSSK
CRRCRLCDEG HGLEVEINCT RTQNTKCRCK PNFFCNSTVC EHCDPCTKCE
HGIIKECTLT SNTKCKEEGS RSNLGWLCLL LLPIPLIVWV KRKEVQKTCR
KHRKENQGSH ESPTLNPETV AINLSDVDLS KYITTIAGVM TLSQVKGFVR
KNGVNEAKID EIKNDNVQDT AEQKVQLLRN WHQLHGKKEA YDTLIKDLKK
ANLCTLAEKI QTIILKDITS DSENSNFRNE IQSLV
UCSC uc001kfr.4; human. [P25445-1];

Category

Human FAS ELISA Kit