Human CDKN2AIPNL ELISA Kit

Alternative name
Human CDKN2A-interacting protein N-terminal-like protein ELISA Kit;Human CDKN2A interacting protein N-terminal like ELISA Kit;Human CDKN2AIP N-terminal-like protein ELISA Kit;
Catalog
E007606
species
Human
GeneCDKN2AIPNL
SpecificityThis assay has high sensitivity and excellent specificity for detection of CDKN2AIPNL. No significant cross-reactivity or interference between CDKN2AIPNL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDKN2AIPNL and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman CDKN2AIPNL ELISA Kit allows for the in vitro quantitative determination of CDKN2AIPNL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This CDKN2AIPNL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDKN2AIPNL. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CDKN2AIPNL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDKN2AIPNL antibody and an CDKN2AIPNL-HRP conjugate. The assay sample and buffer are incubated together with CDKN2AIPNL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDKN2AIPNL concentration since CDKN2AIPNL from samples and CDKN2AIPNL-HRP conjugate compete for the anti-CDKN2AIPNL antibody binding site. Since the number of sites is limited, as more sites are occupied by CDKN2AIPNL from the sample, fewer sites are left to bind CDKN2AIPNL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDKN2AIPNL concentration in each sample is interpolated from this standard curve.
Human CDKN2AIP N-terminal-like protein Protein information
Uniprot ID C2AIL_HUMAN
Uniprot AC Q96HQ2; Q8WVE3;
UniGene Hs.156506;
GeneID 91368
KEGG hsa:91368;
Human CDKN2AIP N-terminal-like protein Protein SEQUENCE
SEQUENCE 116 AA; 13196 MW; 26166C4274FE679C CRC64;
MVGGEAAAAV EELVSGVRQA ADFAEQFRSY SESEKQWKAR MEFILRHLPD
YRDPPDGSGR LDQLLSLSMV WANHLFLGCS YNKDLLDKVM EMADGIEVED
LPQFTTRSEL MKKHQS
UCSC uc011cxs.3; human. [Q96HQ2-1];

Category

Human CDKN2AIPNL ELISA Kit

Human CDKN2A-interacting protein N-terminal-like protein ELISA Kit;Human CDKN2A interacting protein N-terminal like ELISA Kit;Human CDKN2AIP N-terminal-like protein ELISA Kit;